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Proline (Pro) Colorimetric Assay Kit

Cat:E-BC-K177-S Citations (1)
Manual MSDS

Price: $ 360

Price: $ 240

Size:
100Assays 50Assays
Quantity:
  • Sample Type: plant tissue;honey
  • Detection Instrument: Spectrophotometer(520 nm)
  • Reasearch Areas: Amino acids proteins;Plant Physiology Series
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Product Details
Detection Principle Proline can react with acidic-ninhydrin to form stable red compound. The maximum absorption peak of the compound is at 520 nm. And the concentration of Pro can be calculated by measuring the OD value at 520 nm.
Synonyms Pro
Sample Type plant tissue,honey
Sensitivity 0.17 μg/mL
Detection Range 0.17-35 μg/mL
Detection Method Colorimetric method
Assay Time 80 min
Precision inter-assay CV: 5.4% | intra-assay CV: 4.5%
Other Reagents Required Acetic acid, Methylbenzene, Absolute ethyl alcohol
Storage This product can be stored at 2~8°C for 12 months with shading light.
Valid Period 12 months
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Dilution Of Sample

The recommended dilution factor for different samples is as follows (for reference only):

Sample type Dilution factor
Green pepper tissue homogenization 1
Carrot tissue homogenization 1
Epipremnum aureum tissue homogenization 1
Lettuce leaf tissue homogenization 1
Honey 1
Cabbages tissue homogenization 1
The diluent is extracting solution. For the dilution of other sample types, please do pretest to confirm the dilution factor.