For research use only.
| Detection Principle | Glutamic acid can react with NAD<sup>+</sup> under the catalysis of glutamate dehydrogenase to produce α-ketoglutaric acid, NADH and NH<sup>4+</sup>. NADH has the maximum absorption at 340 nm. And glutamic acid content can be calculated by measuring the change of NADH. |
| Sample Type | Serum,Plasma,Animal tissue,Cell,Cell culture supernatant |
| Detection Method | Colorimetric method |
| Detection Instrument | Spectrophotometer (340 nm) |
| Research Area | Amino Acids And Proteins , Ferroptosis |
| Other Reagents Required | Normal saline (0.9% NaCl), PBS (0.01 mol/L, pH 7.4) |
| Storage | This product can be stored at 2-8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components. |
| Valid Period | 12 months |
| Sensitivity | 4.00 μmol/L |
| Detection Range | 4.00-450 μmol/L |
| Precision | inter-assay CV: 2.9% | intra-assay CV: 2.2% |
| Assay Time | 1 h |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| Human serum | 1 |
| Mouse serum | 1 |
| 10% Rat liver tissue homogenate | 1 |
| 10% Rat kidney tissue homogenate | 1 |
| 10% Rat brain tissue homogenate | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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