Protein Thiol Colorimetric Assay Kit (E-BC-K1403-M)
For research use only.
| Detection Principle | Protein thiol are highly abundant in living organisms and play critical roles in diverse physiological processes. Therefore, quantifying their levels in tissues and blood is of significant value for understanding the pathogenesis, treatment, prevention, and prognosis of neurodegenerative diseases. |
| Sample Type | Serum,Plasma,Animal tissue,Cell |
| Detection Method | Colorimetric method |
| Detection Instrument | Microplate reader (405-415 nm) |
| Other Reagents Required | Double distilled water |
| Storage | This product can be stored at -20°C for 12 months with shading light. |
| Valid Period | 12 months |
| Sensitivity | 0.007 mmol/L |
| Detection Range | 0.007-0.5 mmol/L |
| Precision | inter-assay CV: 8.1%-9.8% | intra-assay CV: 2.8%-3.9% |
| Sample Volume | 500 μL |
| Assay Time | 1 h 30 min |
The recommended dilution factor for different samples is as follows (for reference only):
| Sample Type | Dilution Factor |
|---|---|
| 10% Mouse liver tissue homogenization | 2-3 |
| 10% Mouse kidney tissue homogenization | 2-3 |
| 10% Mouse heart tissue homogenization | 2-3 |
| 10% Mouse spleen tissue homogenization | 2-3 |
| 10% Mouse muscle tissue homogenization | 2-3 |
| Rats serum | 1 |
| Mouse serum | 1 |
| Human serum | 1 |
| Human plasma | 1 |
| 1×10^6 RAW264.7cells | 1 |
| 1×10^6 HepG2 cells | 1 |
| 1×10^6 Jurkat cells | 1 |
The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4). For the dilution of other sample types, please do pretest to confirm the dilution factor.
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