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Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Purified Anti-Human CD34 Antibody[4H11]

Purified Anti-Human CD34 Antibody[4H11]

Uniprot : P28906

  • Cat.No.:E-AB-F1324A

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

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  • 25μg
  • 100μg
Price: $50
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Antigen Details

Alternate Names Gp105-120, My10
Swissprot P28906
Gene ID 947
References
1. Stocks SC, et al. 1990. Biochem. J. 268:275.

Product Details

Form Liquid
Clone No. 4H11(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793A]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Unconjugated

How to select the appropriate detection channel through the spectrogram?

Background

CD34, also known as gp105-120, is a type I monomeric sialomucin-like glycophosphoprotein with an approximate molecular weight of 105-120 kD. Selectively expressed on the majority of hematopoietic stem/progenitor cells, bone marrow stromal cells, capillary endothelial cells, embryonic fibroblasts, and some nervous tissue, CD34 is a commonly used marker to identify human hematopoietic stem/progenitor cells. According to the differential sensitivity to enzymatic cleavage, four groups of epitopes of CD34 have been described. CD34 mediates cell adhesion and lymphocytes homing through binding to L-selectin and E-selectin ligands.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

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