Alternate Names	Lymphocyte function-associated antigen 3,CD58,Ag3,Surface glycoprotein LFA-3,LFA3
Swissprot	P19256
References	1. Springer TA, et al. 1987. Annu. Rev. Immunol. 5:223. 2. Dustin ML, et al. 1987. Nature 329:846. 3. Arulanandam AR, et al. 1994. J. Exp. Med. 180:1861. 4. Sanders ME, et al. 1988. J. Immunol. 140:1401.

Product Details

Form	Liquid
Clone No.	TS2/9.1(See Other Available Formats)
Host	Mouse
Isotype	Mouse IgG1, κ
Isotype Control	Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793A]
Reactivity	Human
Application	FCM (See the Application Description)
Storage Buffer	Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer.
Recommended Use	Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 10⁶ cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping	Biological ice pack at 4℃
Stability & Storage	Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase.
Conjugation	Unconjugated

How to select the appropriate detection channel through the spectrogram?

Background

CD58, also known as lymphocyte function-associated antigen 3 (LFA-3) is a 45-70 kD cell surface protein that is a member of the immunoglobulin superfamily. Alternative splicing of CD58 gives rise to transmembrane and glycosylphosphatidylinositol (GPI)-anchored forms on cell surface. CD58 is expressed on both hematopoietic and non-hematopoietic cells including B cells, T cells, monocytes, erythrocytes, endothelial cells, epithelial cells, and fibroblasts. High levels are observed on memory T cells and dendritic cells. CD58 expressed on antigen presenting cells and target cells enhances T cell recognition via the binding of it's cognate ligand, CD2, on the T cell surface.

Protocols & Troubleshootings

Related Protocols	Staining Cell Surface Targets for Flow Cytometry
Troubleshooting	Troubleshooting for Flow Cytometry

Alternative Formats

Product Name	Sizes	Price
Biotin Anti-Human CD58 Antibody[TS2/9.1]	25μg, 100μg	Check More
PE Anti-Human CD58 Antibody[TS2/9.1]	20Tests, 100Tests, 100Tests×2	Check More
APC Anti-Human CD58 Antibody[TS2/9.1]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine5 Anti-Human CD58 Antibody[TS2/9.1]	20Tests, 100Tests, 100Tests×2	Check More
PE/Cyanine7 Anti-Human CD58 Antibody[TS2/9.1]	20Tests, 100Tests, 100Tests×2	Check More
Elab Fluor^® 488 Anti-Human CD58 Antibody[TS2/9.1]	20Tests, 100Tests, 100Tests×2	Check More
PE/Elab Fluor^® 594 Anti-Human CD58 Antibody[TS2/9.1]	20Tests, 100Tests, 100Tests×2	Check More

Citations

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