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Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Purified Anti-Human CD8a Antibody[HIT8a]

Purified Anti-Human CD8a Antibody[HIT8a]

Uniprot : P01732

  • Cat.No.:E-AB-F1271A

  • Applications: FCM
  • Isotype: Mouse IgG1, κ
  • Host: Mouse

To Purchase E-AB-F1271A

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  • 25μg
  • 100μg
Price: $24
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Antigen Details

Alternate Names T-cell surface glycoprotein CD8 alpha chain,CD8A,T8,Leu2,MAL
Swissprot P01732
Gene ID 925
References
1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
2. Knapp W. 1989. Leucocyte Typing IV. Oxford University Press New York.
3. Barclay N, et al. 1997. The Leucocyte Antigen Facts Book. Academic Press Inc. San Diego.
4. Awasthi, S., et al. 2011. J. Virol 85:10472.
5. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
6. Coppieters KT, et al. 2012. J. Exp. Med. 209:51. (IHC, epitope)
7. Suzuki F, et al. 2012. Arthritis Res. Ther. 14:R48. (IHC)

Product Details

Form Liquid
Clone No. HIT8a(See Other Available Formats)
Host Mouse
Isotype Mouse IgG1, κ
Isotype Control Purified Mouse IgG1, κ Isotype Control[MOPC-21] [Product E-AB-F09793A]
Reactivity Human
Application FCM (See the Application Description)
Storage Buffer Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer.
Recommended Use Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Shipping Biological ice pack at 4℃
Stability & Storage Keep as concentrated solution.
Store at 2~8°C. Do not freeze.
Centrifuge before opening to ensure complete recovery of vial contents.
This product is guaranteed up to one year from purchase.
Conjugation Unconjugated

How to select the appropriate detection channel through the spectrogram?

Background

CD8a is a 32-34 kD type I glycoprotein. It forms a homodimer (CD8a/a) or heterodimer (CD8a/b) with CD8b. CD8, also known as T8 and Leu2, is a member of the immunoglobulin superfamily found on the majority of thymocytes, a subset of peripheral blood T cells, and NK cells (which express almost exclusively CD8a homodimers). CD8 acts as a co-receptor with MHC class I-restricted T cell receptors in antigen recognition and T cell activation and has been shown to play a role in thymic differentiation. Two domains in CD8a are important for function: the extracellular IgSF domain binds the α3 domain of MHC class I and the cytoplasmic CXCP motif binds the tyrosine kinase p56 Lck.

Protocols & Troubleshootings

Related Protocols Staining Cell Surface Targets for Flow Cytometry
Troubleshooting

Troubleshooting for Flow Cytometry

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