Current Position:Home>>Antibodies>>Flow Cytometry Antibodies>> Purified Anti-Mouse CD3 Antibody[17A2] E-AB-F1013A
Cat.No.:E-AB-F1013A
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Alternate Names | T-cell surface glycoprotein CD 3epsilon/delta/gamma/zeta chain,CD3E/D/G/Z,CD3e/d/g/z,CD3E/D/G/Z,CD3 |
Swissprot | P04235,P11942,P22646,P24161 |
References |
1. Barclay A, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Davis MM. 1990. Annu. Rev. Biochem. 59:475. 3. Weiss A, et al. 1994. Cell 76:263. |
Form | Liquid |
Clone No. | 17A2(See Other Available Formats) |
Host | Rat |
Isotype | Rat IgG2b, κ |
Isotype Control | Purified Rat IgG2b, κ Isotype Control[LTF-2] [Product E-AB-F09843A] |
Reactivity | Mouse |
Application | FCM (See the Application Description) |
Storage Buffer | Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer. |
Recommended Use | Each lot of this antibody is quality control tested by flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106 cells in 100 µL volume or 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application. |
Shipping | Biological ice pack at 4℃ |
Stability & Storage | Keep as concentrated solution. Store at 2~8°C. Do not freeze. Centrifuge before opening to ensure complete recovery of vial contents. This product is guaranteed up to one year from purchase. |
Conjugation | Unconjugated |
How to select the appropriate detection channel through the spectrogram?
CD3, also known as T3, is a member of the Ig superfamily and primarily expressed on T cells, NK-T cells, and at different levels on thymocytes during T cell differentiation. CD3 is composed of CD3ε, δ, γ and ζ chains. It forms a TCR complex by associating with TCR α/β or γ/δ chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. |
Related Protocols | Staining Cell Surface Targets for Flow Cytometry |
Troubleshooting |
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