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Pyruvic Acid Colorimetric Assay Kit

  • Cat.No.:E-BC-K130-M

  • Detection instrument: Microplate reader(480-520 nm,optimum wavelength: 505 nm)

To Purchase E-BC-K130-M

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  • 96T
  • 48T
Price: $220
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Detection principle

Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm.

Performance characteristics

Sample type Serum,plasma,tissue
Sensitivity 0.003 μmol/mL
Detection range 0.003-2.0 μmol/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 3.6%Average intra-assay CV: 2.3%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.003-2.0 μmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum

1

Mouse serum

1

Mouse plasma

1

10% Mouse liver tissue homogenate

1

10% Rat kidney tissue homogenate

1

10% Rat heart tissue homogenate

1

Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

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