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Pyruvic Acid Colorimetric Assay Kit

  • Cat.No.:E-BC-K130-M

  • Detection instrument: Microplate reader(480-520 nm,optimum wavelength: 505 nm)

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Price: $220

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Detection principle

Pyruvic acid can react with chromogenic agent and the reaction product is reddish brown in alkaline solution. The depth of color is directly proportional to the pyruvate content. The pyruvate content can be calculated by measuring the OD value at 505 nm.

Performance characteristics

Sample type Serum,plasma,tissue
Sensitivity 0.003 μmol/mL
Detection range 0.003-2.0 μmol/mL
Detection method Colorimetric method
Assay type Quantitative
Assay time 30 min
Precision Average inter-assay CV: 3.6%Average intra-assay CV: 2.3%
Other instruments required Micropipettor, Centrifuge, Incubator, Vortex mixer
Other reagents required Normal saline (0.9% NaCl), PBS (0.01 M, pH 7.4)
Storage 2-8℃
Valid period 6 months

Dilution of sample

It is recommended to take 2~3 samples with expected large difference to do pre-experiment before formal experiment and dilute the sample according to the result of the pre-experiment and the detection range (0.003-2.0 μmol/mL).

The recommended dilution factor for different samples is as follows (for reference only):

Sample type

Dilution factor

Human serum


Mouse serum


Mouse plasma


10% Mouse liver tissue homogenate


10% Rat kidney tissue homogenate


10% Rat heart tissue homogenate


Note: The diluent is normal saline (0.9% NaCl) or PBS (0.01 M, pH 7.4).


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