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QuicKey Pro Human T(Testosterone)ELISA Kit

Cat.No.:E-OSEL-H0007
Reactivity: Human

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96T
48T
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96T*5
96T*10

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QuicKey Pro Series

Get more sensitive and precise results with saving at least 1-2h comparing to traditional ELISA Kits. The new developed technology in house will help to accelerate your science research in a more efficient way.

Product Details

Properties

Assay type	Competitive-ELISA
Format	96T/48T/24
Assay time	1.5h
Detection range	0.63-40 ng/mL
Sensitivity	0.24 ng/mL
Sample type &Sample volume	serum, plasma, urine, saliva; 50μL
Specificity	This kit recognizes T in samples. No significant cross-reactivity or interference between T and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of T concentrations in serum, plasma, urine, saliva.

Test Principle

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Human T. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Human T are added to the micro ELISA plate wells. Human T in samples (or standards) competes with a fixed amount of T on the solid phase supporter for sites on the HRP linked detection antibody specific to T. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Human T in the samples is then determined by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 6 months. After test, the unused wells and reagents should be stored according to the table.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips 24T: 8 wells ×3 strips 96T*5: 5 plates, 96T	2-8℃, 1 months
Reference Standard	96T: 2 vials 48T/24T: 1 vial 96T*5: 10 vials	2-8℃, use the reconstituted standard within 24h
Concentrated HRP Linked Detection Ab(100×)	96T: 1 vial, 60 μL 48T/24T: 1 vial, 30 μL 96T*5: 5 vials, 60 μL	2-8℃(Protect from light)
Reference Standard & Sample Diluent	96T/48T/24T: 1 vial, 20 mL 96T*5: 5 vials, 20 mL	2-8℃
HRP Linked Ab Diluent	96T/48T/24T: 1 vial, 14 mL 96T*5: 5 vials, 14 mL
Concentrated Wash Buffer(25×)	96T/48T/24T: 1 vial, 30 mL 96T*5: 5 vials, 30 mL
Substrate Reagent	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃(Protect from light)
Stop Solution	96T/48T/24T: 1 vial, 10 mL 96T*5: 5 vials, 10 mL	2-8℃
Plate Sealer	96T/48T/24T: 5 pieces 96T*5: 25 pieces
Product Description	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level T were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level T were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	2.04	4.88	18.80	2.01	4.96	18.17
Standard deviation	0.14	0.24	0.80	0.12	0.25	0.80
CV (%)	6.63	4.93	4.27	6.08	5.07	4.38

Recovery

The recovery of T spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	86-99	91
EDTA plasma(n=8)	94-106	100
Urine(n=8)	93-109	99

Linearity

Samples were spiked with high concentrations of Human T and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	Testosterone，T
Research Area	Signal Transduction, Signal Transduction, Epigenetics and Nuclear Signaling, Developmental Biology

Assay Procedures

Citations

TOXICOLOGY LETTERS (2021) IF: 4.374
Copper exposure disrupts ovarian steroidogenesis in human ovarian granulosa cells via the FSHR/CYP19A1 pathway and alters methylation patterns on the SF-1 gene promoter

DOI: 10.1016/j.toxlet.2021.12.002

PMID: 34871762
Sample: ovarian granulosa cell
Frontiers in Physiology (2021) IF: 4.566
Human Physiological Responses to a Single Deep Helium-Oxygen Diving

DOI: 10.3389/fphys.2021.735986

Sample: saliva
NITRIC OXIDE-BIOLOGY AND CHEMISTRY (2020) IF: 3.311
Estrogen receptor (ESR1 and ESR2)-mediated activation of eNOS–NO–cGMP pathway facilitates high altitude acclimatization

DOI: 10.1016/j.niox.2020.05.003

Sample: Plasma
TOXICOLOGY LETTERS (2019) IF: 3.499
Cholestasis-associated reproductive toxicity in male and female rats: The fundamental role of mitochondrial impairment and oxidative stress

DOI: 10.1016/j.toxlet.2019.09.009

Sample: serum
TOXICOLOGY AND APPLIED PHARMACOLOGY (2019) IF: 3.585
High copper levels in follicular fluid affect follicle development in polycystic ovary syndrome patients: Population-based and in vitro studies

DOI: 10.1016/j.taap.2019.01.008

PMID: 30641075
Sample: Serum,Cell culture medium
REPRODUCTION (2021) IF: 3.906
Melatonin ameliorates ovarian dysfunction by regulating autophagy in PCOS via the PI3K-Akt pathway

DOI: 10.1530/REP-20-0643

PMID: 33989172
Sample: serum
Reproductive Biology (2022) IF: 2.089
ALG2 inhibits the epithelial-to-mesenchymal transition and stemness of ovarian granulosa cells through the Wnt/β-catenin signaling pathway in polycystic ovary syndrome

DOI: 10.1016/j.repbio.2022.100706

PMID: 36327672
Sample: serum
ANDROLOGIA (2022) IF: 2.532
The impact of dolutegravir-based combination antiretroviral therapy on the spermatozoa and fertility parameters of men living with human immunodeficiency virus

DOI: 10.1111/and.14621

PMID: 36261884
Sample: serum
HIGH ALTITUDE MEDICINE & BIOLOGY (2021) IF: 1.981
Association Between 17β-Estradiol Receptors and Nitric Oxide Signaling Augments High-Altitude Adaptation of Ladakhi Highlanders

DOI: 10.1089/ham.2020.0187

Sample: plasma

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