Rabbit IgG(Immunoglobulin G) ELISA Kit (E-EL-RB2288)

This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Rabbit IgG. During the reaction, Rabbit IgG in the sample or standard competes with a fixed amount of Rabbit IgG on the solid phase supporter for sites on the Biotinylated Detection Ab specific to Rabbit IgG. Excess conjugate and unbound sample or standard are washed away, and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns from blue to yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of Rabbit IgG in tested samples can be calculated by comparing the OD of the samples to the standard curve.

Immunoglobulin G is the main component of serum immunoglobulin, accounting for about 75% of the total content of serum immunoglobulin, and the normal value is 9.5~12.5mg无ml. Of these, 40-50% are distributed in the serum, and the rest are distributed in the tissue. The molecular weight is about 150,000 Daltons. Because IgG can cross the placenta, the IgG acquired by the newborn from the mother plays an important role in fighting infection. IgG synthesis begins 2 to 4 weeks after birth, and serum IgG can reach adult levels after 8 years of age. Because IgG is more easily diffused into the space outside blood vessels than other Ig, it plays an important role in binding complement, enhancing the ability of immune cells to phagocytic pathogenic microorganisms and neutralizing bacterial toxins, and can effectively fight infection, which is a favorable side for human body. However, in some autoimmune diseases, such as autoimmune hemolytic anemia, thrombocytopenic purpura, lupus erythematosus and rheumatoid disease, the autoantibodies are IgG. Once it binds to the corresponding own cells, it instead enhances the tissue damage effect.