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Rat ACE(Angiotensin Ⅰ Converting Enzyme) ELISA Kit

Cat.No.:E-EL-R2401
Reactivity: Rat

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat ACE in samples. No significant cross-reactivity or interference between Rat ACE and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat ACE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ACE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ACE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ACE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ACE. You can calculate the concentration of Rat ACE in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ACE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ACE were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.10	3.06	9.84	1.20	3.18	9.23
Standard deviation	0.06	0.13	0.31	0.07	0.18	0.30
CV (%)	5.45	4.25	3.15	5.83	5.66	3.25

Recovery

The recovery of Rat ACE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-107	101
EDTA plasma (n=8)	95-111	102
Cell culture media (n=8)	87-103	94

Linearity

Samples were spiked with high concentrations of Rat ACE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	ACE1, CD143, DCP, DCP1, ICH, MVCD3
Research Area	Cancer, Cell Biology, Cardiovascular, Metabolism, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
Kolaviron attenuates cardiovascular injury in fructose-streptozotocin induced type-2 diabetic male rats by reducing oxidative stress, inflammation, and improving cardiovascular risk markers

DOI: 10.1016/j.biopha.2021.112323

PMID: 34656062
Sample: serum
Frontiers in Pharmacology (2021) IF: 5.811
Centella asiatica (L.) Urb. Prevents Hypertension and Protects the Heart in Chronic Nitric Oxide Deficiency Rat Model.

DOI: 10.3389/fphar.2021.742562

Sample: Cardiac tissue
Oxidative Medicine and Cellular Longevity (2018) IF: 4.936
Antihypertensive Effect of Ethanolic Extract from Acanthopanax sessiliflorus Fruits and Quality Control of Active Compounds

DOI: 10.1155/2018/5158243

Sample: serum
LIFE SCIENCES (2020) IF: 3.647
Taxifolin improves disorders of glucose metabolism and water-salt metabolism in kidney via PI3K/AKT signaling pathway in metabolic syndrome rats

DOI: 10.1016/j.lfs.2020.118713

PMID: 33157091
Sample: Serum,Urine
JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2023) IF: 3.993
Buchholzia coriacea seed induce antifertility by interfering with steroidogenic enzymes and inflammatory cytokines in rat testis

DOI: 10.1016/j.jri.2023.103923

Sample: Testicular
MOLECULAR BIOLOGY REPORTS (2019) IF: 2.107
var kunstleri in spontaneously hypertensive rats: possible involvement of renin–angiotensin–aldosterone system, endothelial function and anti-oxidant system

DOI: 10.1007/s11033-019-04730-w

Sample: serum,urine
CHRONOBIOLOGY INTERNATIONAL (2019) IF: 2.562
Valsartan chronotherapy reverts the non-dipper pattern and improves blood pressure control through mediation of circadian rhythms of the renin-angiotensin system in spontaneous hypertension rats

DOI: 10.1080/07420528.2019.1610419

Sample: plasma
ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575
Angiotensin converting enzyme inhibitor potentiates the hypoglycaemic effect of NG-nitro-L-arginine methyl ester (L-NAME) in rats

DOI: 10.1080/13813455.2020.1780263

PMID: 32584611
Sample: Serum
MOLECULES (2017) IF: 2.861
Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

DOI: 10.3390/molecules22111956

Sample: Cell culture supernatant
PHARMACEUTICAL BIOLOGY (2020) IF: 2.971
Effect of phenylacetamide isolated from lepidium apetalum on myocardial injury in spontaneously hypertensive rats and its possible mechanism

DOI: 10.1080/13880209.2020.1778043

Sample: myocardial tissue
CLINICAL AND EXPERIMENTAL HYPERTENSION (2019) IF: 1.522
Standardized ethanol-water extract of Ficus deltoidea Angustifolia reduces blood pressure in spontaneously hypertensive rats

DOI: 10.1080/10641963.2018.1506467

PMID: 30648895
Sample: Serum
Physiological Reports (2023)
COVID-19: Understanding the impact of anti-hypertensive drugs and hydroxychloroquine on the ACE1 and ACE2 in lung and adipose tissue in SHR and WKY rats

DOI: 10.14814/phy2.15598

Sample: Serum

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