Rat AChE (Acetylcholinesterase) CLIA Kit (E-CL-R0230)

Name: Rat AChE (Acetylcholinesterase) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0230
Price: 180.0 USD
Availability: InStock

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Rat AChE (Acetylcholinesterase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat AChE in samples.No significant cross-reactivity or interference between Rat AChE and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	30672.000	34618.000	32645.000	32604
5	16945.000	16993.000	16969.000	16928
2.5	8272.000	6980.000	7626.000	7585
1.25	3735.000	3885.000	3810.000	3769
0.63	1782.000	1758.000	1770.000	1729
0.31	931.000	807.000	869.000	828
0.16	374.000	402.000	388.000	347
0	40.000	42.000	41.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.540	1.250	4.990	0.530	1.270	4.710
Standard deviation	0.050	0.090	0.510	0.070	0.090	0.490
CV（%）	9.260	7.200	10.220	13.210	7.090	10.400

Recovery

The recovery of Rat AChE was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-106	100
EDTA plasma （n=8）	88-99	93
Cell culture media （n=8）	94-107	101

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat AChE and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-105	85-100	96-111
1:2	Average	98	92	101
1:4	Range	93-109	88-102	84-97
1:4	Average	100	94	91
1:8	Range	89-101	88-102	89-101
1:8	Average	95	95	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AChE. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AChE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AChE, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat AChE. You can calculate the concentration of Rat AChE in the samples by comparing the RLU value of the samples to the standard curve.

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