Rat AhR (Aryl Hydrocarbon Receptor) CLIA Kit (E-CL-R0058)

Name: Rat AhR (Aryl Hydrocarbon Receptor) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0058
Price: 180.0 USD
Availability: InStock

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Rat AhR (Aryl Hydrocarbon Receptor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat AhR in samples.No significant cross-reactivity or interference between Rat AhR and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	55181.000	63525.000	59353.000	59333
2000	28764.000	28920.000	28842.000	28822
1000	14918.000	14136.000	14527.000	14507
500	6657.000	6851.000	6754.000	6734
250	3547.000	3085.000	3316.000	3296
125	1729.000	1687.000	1708.000	1688
62.5	758.000	758.000	758.000	738
0	19.000	21.000	20.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	202.280	452.430	1658.420	220.530	429.080	1823.820
Standard deviation	23.060	42.210	180.930	24.130	48.270	121.470
CV（%）	11.400	9.330	10.910	10.940	11.250	6.660

Recovery

The recovery of Rat AhR was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	86-100	93
EDTA plasma （n=8）	100-113	106
Cell culture media （n=8）	87-98	92

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat AhR and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-108	88-101	101-118
1:2	Average	100	95	107
1:4	Range	102-116	99-110	85-97
1:4	Average	110	104	91
1:8	Range	84-97	96-109	99-115
1:8	Average	91	102	105

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AhR. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AhR and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AhR, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat AhR. You can calculate the concentration of Rat AhR in the samples by comparing the RLU value of the samples to the standard curve.

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