Rat AMH (Anti-Mullerian Hormone) CLIA Kit (E-CL-R0463)

Name: Rat AMH (Anti-Mullerian Hormone) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0463
Price: 180.0 USD
Availability: InStock

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Rat AMH (Anti-Mullerian Hormone) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat AMH in samples.No significant cross-reactivity or interference between Rat AMH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	57815.000	61393.000	59604.000	59573
1000	25035.000	27059.000	26047.000	26016
500	13057.000	10977.000	12017.000	11986
250	5561.000	5851.000	5706.000	5675
125	3044.000	2620.000	2832.000	2801
62.5	1459.000	1257.000	1358.000	1327
31.25	715.000	721.000	718.000	687
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	99.330	195.400	990.020	106.120	191.420	1043.020
Standard deviation	10.040	14.230	109.790	10.030	20.850	109.000
CV（%）	10.110	7.280	11.090	9.450	10.890	10.450

Recovery

The recovery of Rat AMH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-106	101
EDTA plasma （n=8）	99-111	104
Cell culture media （n=8）	100-113	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat AMH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-102	95-113	87-100
1:2	Average	94	103	94
1:4	Range	88-103	90-105	92-108
1:4	Average	95	98	99
1:8	Range	91-108	92-105	93-104
1:8	Average	99	99	99

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat AMH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat AMH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat AMH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat AMH. You can calculate the concentration of Rat AMH in the samples by comparing the RLU value of the samples to the standard curve.

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