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Rat BAX(Bcl-2 Associated X Protein) ELISA Kit

Citations(20)

  • Cat.No.:E-EL-R0098

  • Reactivity: Rat
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Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat BAX in samples. No significant cross-reactivity or interference between Rat BAX and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat BAX concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BAX. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BAX and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BAX, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BAX. You can calculate the concentration of Rat BAX in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BAX were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BAX were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.49 1.35 3.37 0.49 1.30 3.07
Standard deviation 0.03 0.07 0.14 0.03 0.07 0.14
CV (%) 6.12 5.19 4.15 6.12 5.38 4.56

Recovery

The recovery of Rat BAX spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-103 95
EDTA plasma (n=8) 95-111 101
Cell culture media (n=8) 90-102 96

Linearity

Samples were spiked with high concentrations of Rat BAX and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsBCL2L4
Research AreaCancer, Cell Biology, Metabolism

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

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Citations

  1. International Journal of Nanomedicine (2021) IF: 6.4
    Neuroprotective Efficiency of Prodigiosins Conjugated with Selenium Nanoparticles in Rats Exposed to Chronic Unpredictable Mild Stress is Mediated Through Antioxidative, Anti-Inflammatory, Anti-Apoptotic, and Neuromodulatory Activities

    DOI: 10.2147/IJN.S323436

    PMID: 35002238

    		 Sample: Hippocampal Tissue
  2. SCIENCE OF THE TOTAL ENVIRONMENT (2020) IF: 6.551
    Protocatechuic acid mitigates cadmium-induced neurotoxicity in rats: Role of oxidative stress, inflammation and apoptosis

    DOI: 10.1016/j.scitotenv.2020.137969
    Sample: cortical
  3. LIFE SCIENCES (2022) IF: 6.78
    Irbesartan reprofiling for the amelioration of ethanol-induced gastric mucosal injury in rats: Role of inflammation, apoptosis, and autophagy

    DOI: 10.1016/j.lfs.2022.120939

    PMID: 36115582

    		 Sample: gastric tissue
  4. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH (2023) IF: 5.19
    Betulinic acid protects against cardiotoxicity of the organophosphorus pesticide chlorpyrifos by suppressing oxidative stress, inflammation, and apoptosis in rats

    DOI: 10.1007/s11356-023-25917-6
    Sample: heart
  5. Pharmaceuticals (2022) IF: 5.215
    Lycopene Modulates Oxidative Stress and Inflammation in Hypercholesterolemic Rats

    DOI: 10.3390/ph15111420
    Sample: Cardiac,Renal
  6. SAUDI JOURNAL OF BIOLOGICAL SCIENCES (2021) IF: 4.219
    Neuroprotective efficacy of 4-Hydroxyisoleucine in experimentally induced intracerebral hemorrhage

    DOI: 10.1016/j.sjbs.2021.07.010

    PMID: 34764759

    		 Sample: brain
  7. TOXICOLOGY (2021) IF: 4.221
    3-Indolepropionic acid upturned male reproductive function by reducing oxido-inflammatory responses and apoptosis along the hypothalamic-pituitary-gonadal axis of adult rats exposed to chlorpyrifos

    DOI: 10.1016/j.tox.2021.152996

    PMID: 34678318

    		 Sample: testes,epididymis,hypothalamus
  8. MOLECULES (2022) IF: 4.412
    Guggulsterone Mediated JAK/STAT and PPAR-Gamma Modulation Prevents Neurobehavioral and Neurochemical Abnormalities in Propionic Acid-Induced Experimental Model of Autism

    DOI: 10.3390/molecules27030889
    Sample: brain,blood plasma
  9. NEUROTOXICOLOGY (2018) IF: 3.076
    Antiapoptotic effect of taurine against NMDA-induced retinal excitotoxicity in rats

    DOI: 10.1016/j.neuro.2018.10.009

    PMID: 30385388

    		 Sample: Tissue homogenate
  10. NEUROTOXICITY RESEARCH (2016) IF: 3.14
    Neuroprotective Effect of Magnesium Acetyltaurate Against NMDA-Induced Excitotoxicity in Rat Retina

    DOI: 10.1007/s12640-016-9658-9
    Sample: Tissue homogenate
  11. METABOLIC BRAIN DISEASE (2020) IF: 3.584
    Curcumin attenuates cytoplasmic/endoplasmic reticulum stress, apoptosis and cholinergic dysfunction in diabetic rat hippocampus

    DOI: 10.1007/s11011-020-00551-0

    PMID: 32172517

    		 Sample: Tissue homogenate
  12. JOURNAL OF FOOD BIOCHEMISTRY (2022) IF: 3.654
    Twelve weeks of treadmill exercise training with green tea extract reduces myocardial oxidative stress and alleviates cardiomyocyte apoptosis in aging rat: The emerging role of BNIP3 and HIF-1α/IGFBP3 pathway

    DOI: 10.1111/jfbc.14397

    PMID: 36069470

    		 Sample: heart tissue
  13. BMC Complementary and Alternative Medicine (2022) IF: 3.659
    Neuroprotective effects of Lippia javanica (Burm.F.) Spreng. Herbal tea infusion on Lead-induced oxidative brain damage in Wistar rats

    DOI: 10.1186/s12906-021-03471-3

    PMID: 34983490

    		 Sample: brain tissue
  14. NEUROCHEMICAL RESEARCH (2022) IF: 3.996
    Tetramethylpyrazine Attenuates Cognitive Impairment Via Suppressing Oxidative Stress, Neuroinflammation, and Apoptosis in Type 2 Diabetic Rats

    DOI: 10.1007/s11064-022-03640-x

    PMID: 35665448

    		 Sample: hippocampal tissue
  15. ANDROLOGIA (2021) IF: 2.775
    Decrease in reproductive dysfunction using aflatoxin B1 exposure: a treatment with 3-indolepropionic acid in albino Wistar rat

    DOI: 10.1111/and.14248

    PMID: 34541692

    		 Sample: testicular,epididymal,hypothalamic
  16. NEUROSCIENCE RESEARCH (2023) IF: 2.904
    Neuroprotection by trans-resveratrol in rats with N-methyl-D-aspartate (NMDA)–induced retinal injury: insights into the role of adenosine A1 receptors

    DOI: 10.1016/j.neures.2023.02.004
    Sample: retinal
  17. Molecular Medicine Reports (2022) IF: 2.952
    Propofol postconditioning alleviates diabetic myocardial ischemia‑reperfusion injury via the miR‑200c‑3p/AdipoR2/STAT3 signaling pathway

    DOI: 10.3892/mmr.2022.12653
    Sample: serum
  18. ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS (2020) IF: 1.28
    Involvement of redox status and the nuclear-related factor 2 in protecting against cadmium-induced renal injury with Sana Makki (Cassia senna L.) pre-treatment in male rats

    DOI: 10.1590/0001-3765202020191237
    Sample: Serum,Tissue homogenate
  19. Iranian Journal of Basic Medical Sciences (2018) IF: 1.514
    Sleep deprivation disrupts striatal anti-apoptotic responses in 6-hydroxy dopamine-lesioned parkinsonian rats

    DOI: 10.22038/ijbms.2018.28546.6919

    PMID: 30627374

    		 Sample: Tissue homogenate
  20. Experimental and Therapeutic Medicine (2020) IF: 1.785
    miR‑9 inhibition of neuronal apoptosis and expression levels of apoptosis genes Bcl‑2 and Bax in depression model rats through Notch pathway

    DOI: 10.3892/etm.2019.8228
    Sample: serum
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