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Rat BMP-2(Bone Morphogenetic Protein 2) ELISA Kit

Citations(7)Uniprot : P49001

Cat.No.:E-EL-R0002
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat BMP-2 in samples. No significant cross-reactivity or interference between Rat BMP-2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat BMP-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat BMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat BMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat BMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat BMP-2. You can calculate the concentration of Rat BMP-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat BMP-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat BMP-2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	182.20	368.60	1622.50	178.60	372.20	1493.30
Standard deviation	11.10	15.50	87.60	9.50	21.20	76.20
CV (%)	6.09	4.21	5.40	5.32	5.70	5.10

Recovery

The recovery of Rat BMP-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	93-104	99
EDTA plasma (n=8)	90-104	97
Cell culture media (n=8)	87-97	92

Linearity

Samples were spiked with high concentrations of Rat BMP-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Database Links	SwissProt: P49001
Synonyms	BMP2A, BMP-2A, BDA2
Research Area	Cancer, Cardiovascular, Signal transduction, Stem cells

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

BIOMATERIALS (2022) IF: 15.304
Immunology and bioinformatics analysis of injectable organic/inorganic microfluidic microspheres for promoting bone repair

DOI: 10.1016/j.biomaterials.2022.121685

PMID: 35953327
Sample: BMSCs
Advanced Healthcare Materials (2022) IF: 11.092
Spatiotemporal regulation of the bone immune microenvironment via dam-like biphasic bionic periosteum for bone regeneration

DOI: 10.1002/adhm.202201661

PMID: 36189833
Sample: BMSCs
Bio-Design and Manufacturing (2022) IF: 6.302
Injectable bioactive polymethyl methacrylate–hydrogel hybrid bone cement loaded with BMP-2 to improve osteogenesis for percutaneous vertebroplasty and kyphoplasty

DOI: 10.1007/s42242-021-00172-1

Sample: bone marrow mesenchymal stem cell (BMSCs)
Biomaterials Science (2019) IF: 6.843
ONO-1301 loaded nanocomposite scaffolds modulate cAMP mediated signaling and induce new bone formation in critical sized bone defect

DOI: 10.1039/C9BM01352K

Sample: Mesenchymal stem cell
CHEMOSPHERE (2015) IF: 3.34
Effects of nitrate on metamorphosis, thyroid and iodothyronine deiodinases expression in Bufo gargarizans larvae

DOI: 10.1016/j.chemosphere.2015.07.037

Sample: Tissue homogenate
CLINICAL ORAL IMPLANTS RESEARCH (2020) IF: 3.723
Tibolone, alendronate, and simvastatin enhance implant osseointegration in a preclinical in vivo model

DOI: 10.1111/clr.13602

Sample: blood
CURRENT PHARMACEUTICAL DESIGN (2019) IF: 2.412
Hyaluronic Acid Modified Risedronate and Teriparatide Co-loaded Nanocarriers for Improved Osteogenic Differentiation of Osteoblasts for the Treatment of Osteoporosis

DOI: 10.2174/1381612825666190801140703

Sample: MC3T3-E1 cells

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