Rat CK-MB (Creatine Kinase MB Isoenzyme) CLIA Kit (E-CL-R0722)

Name: Rat CK-MB (Creatine Kinase MB Isoenzyme) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0722
Price: 180.0 USD
Availability: InStock

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Rat CK-MB (Creatine Kinase MB Isoenzyme) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat CK-MB in samples.No significant cross-reactivity or interference between Rat CK-MB and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	49579.000	55973.000	52776.000	52746
400	21183.000	23179.000	22181.000	22151
200	10398.000	9060.000	9729.000	9699
100	4643.000	4679.000	4661.000	4631
50	2461.000	2167.000	2314.000	2284
25	1215.000	1051.000	1133.000	1103
12.5	505.000	521.000	513.000	483
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	43.110	127.930	343.800	44.970	139.700	357.570
Standard deviation	3.720	13.650	38.570	4.580	10.240	37.510
CV（%）	8.630	10.670	11.220	10.180	7.330	10.490

Recovery

The recovery of Rat CK-MB was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-110	104
EDTA plasma （n=8）	92-105	99
Cell culture media （n=8）	89-101	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat CK-MB and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-103	96-108	98-112
1:2	Average	97	103	106
1:4	Range	85-97	100-115	92-106
1:4	Average	91	107	98
1:8	Range	100-114	93-105	90-106
1:8	Average	107	99	97

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CK-MB. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CK-MB and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CK-MB, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CK-MB. You can calculate the concentration of Rat CK-MB in the samples by comparing the RLU value of the samples to the standard curve.

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