Rat CNTF (Ciliary Neurotrophic Factor) CLIA Kit (E-CL-R0143)

Name: Rat CNTF (Ciliary Neurotrophic Factor) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0143
Price: 180.0 USD
Availability: InStock

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Rat CNTF (Ciliary Neurotrophic Factor) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat CNTF in samples.No significant cross-reactivity or interference between Rat CNTF and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	52111.000	55025.000	53568.000	53536
400	23031.000	23807.000	23419.000	23387
200	10625.000	9395.000	10010.000	9978
100	4888.000	4898.000	4893.000	4861
50	2492.000	2312.000	2402.000	2370
25	1157.000	1097.000	1127.000	1095
12.5	506.000	532.000	519.000	487
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	37.350	92.200	304.500	38.820	89.700	308.060
Standard deviation	3.200	8.000	27.340	3.640	7.940	28.310
CV（%）	8.570	8.680	8.980	9.380	8.850	9.190

Recovery

The recovery of Rat CNTF was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-109	100
EDTA plasma （n=8）	95-106	101
Cell culture media （n=8）	96-109	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat CNTF and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-105	91-105	88-102
1:2	Average	97	98	93
1:4	Range	90-105	94-110	101-114
1:4	Average	97	101	107
1:8	Range	92-105	99-116	90-103
1:8	Average	100	107	96

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CNTF. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CNTF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CNTF, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CNTF. You can calculate the concentration of Rat CNTF in the samples by comparing the RLU value of the samples to the standard curve.

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