Rat CREB1 (Cyclic AMP Response Element Binding Protein 1) CLIA Kit (E-CL-R0192)

Name: Rat CREB1 (Cyclic AMP Response Element Binding Protein 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0192
Price: 180.0 USD
Availability: InStock

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Rat CREB1 (Cyclic AMP Response Element Binding Protein 1) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 fmol/mL
Detection Range	62.5-4000 fmol/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat CREB1 in samples.No significant cross-reactivity or interference between Rat CREB1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

fmol/mL	OD1	OD2	Mean OD	Corrected OD
4000	59395.000	63961.000	61678.000	61641
2000	27177.000	32061.000	29619.000	29582
1000	14114.000	13432.000	13773.000	13736
500	6421.000	6989.000	6705.000	6668
250	3572.000	3498.000	3535.000	3498
125	1748.000	1720.000	1734.000	1697
62.5	791.000	869.000	830.000	793
0	35.000	39.000	37.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（fmol/mL）	187.570	625.570	1678.400	202.450	609.190	1761.830
Standard deviation	24.070	55.860	173.880	24.840	51.900	179.530
CV（%）	12.830	8.930	10.360	12.270	8.520	10.190

Recovery

The recovery of Rat CREB1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-101	96
EDTA plasma （n=8）	99-114	106
Cell culture media （n=8）	97-108	103

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat CREB1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	86-98	87-100	97-113
1:2	Average	91	94	104
1:4	Range	93-110	102-117	86-99
1:4	Average	101	109	91
1:8	Range	95-109	91-104	103-118
1:8	Average	102	98	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CREB1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CREB1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CREB1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CREB1. You can calculate the concentration of Rat CREB1 in the samples by comparing the RLU value of the samples to the standard curve.

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