Rat CRT (Calreticulin) CLIA Kit (E-CL-R0101)

Name: Rat CRT (Calreticulin) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0101
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat CRT in samples.No significant cross-reactivity or interference between Rat CRT and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	51524.000	59850.000	55687.000	55658
500	21513.000	26095.000	23804.000	23775
250	10476.000	10404.000	10440.000	10411
125	4787.000	5317.000	5052.000	5023
62.5	2581.000	2383.000	2482.000	2453
31.25	1409.000	1313.000	1361.000	1332
15.63	752.000	766.000	759.000	730
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	49.660	111.140	338.030	49.520	106.520	345.160
Standard deviation	4.480	10.170	32.890	5.460	11.650	35.760
CV（%）	9.020	9.150	9.730	11.030	10.940	10.360

Recovery

The recovery of Rat CRT was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-101	96
EDTA plasma （n=8）	89-103	95
Cell culture media （n=8）	91-104	97

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat CRT and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-102	93-105	91-104
1:2	Average	97	99	97
1:4	Range	103-118	98-115	96-110
1:4	Average	110	106	103
1:8	Range	101-117	89-100	85-95
1:8	Average	108	94	90

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CRT. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CRT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CRT, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CRT. You can calculate the concentration of Rat CRT in the samples by comparing the RLU value of the samples to the standard curve.

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