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Cat.No.:E-CL-R0721
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Assay type | Sandwich-CLIA |
Format | 96T/48T |
Assay time | 3.5h |
Detection range | 12.50-800 pg/mL |
Sensitivity | 7.50 pg/mL |
Sample type &Sample volume | serum, plasma and other biological fluids; 100μL |
Specificity | This kit recognizes Rat cTn-I/TNNI3 in samples. No significant cross-reactivity or interference between Rat cTn-I/TNNI3 and analogues was observed. |
Reproducibility | Both intra-CV and inter-CV are < 15%. |
Application | This CLIA kit applies to the in vitro quantitative determination of Rat cTn-I/TNNI3 concentrations in serum, plasma and other biological fluids. |
Item | Specifications | Storage |
---|---|---|
Micro CLIA Plate(Dismountable) | 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips |
-20℃, 6 months |
Reference Standard | 96T: 2 vials
48T: 1 vial |
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Concentrated Biotinylated Detection Ab (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
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Concentrated HRP Conjugate (100×) | 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL |
-20℃(Protect from light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 2-8°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent A | 1 vial, 5 mL | 2-8℃(Protect from light) |
Substrate Reagent B | 1 vial, 5 mL | |
Plate Sealer | 5 pieces | |
Manual | 1 copy | |
Certificate of Analysis | 1 copy |
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat cTn-I/TNNI3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat cTn-I/TNNI3 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 43.14 | 127.67 | 380.90 | 42.34 | 115.95 | 403.72 |
Standard deviation | 4.31 | 14.45 | 34.93 | 5.40 | 10.41 | 45.38 |
CV (%) | 9.99 | 11.32 | 9.17 | 12.75 | 8.98 | 11.24 |
The recovery of Rat cTn-I/TNNI3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
---|---|---|
Serum(n=8) | 88-103 | 95 |
EDTA plasma(n=8) | 95-109 | 103 |
Cell culture media(n=8) | 89-102 | 96 |
Samples were spiked with high concentrations of Rat cTn-I/TNNI3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Synonyms | TNNC1, cTnI, CMD1FF, CMD2A, CMH7, RCM1 |
Research Area | Cancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells |
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1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃. |
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![]() 2.Remove the liquid. |
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![]() 3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times. |
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![]() 4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times. |
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![]() 5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃. |
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![]() 6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results. |
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Sample: plasma |
DOI: 10.1039/C8RA08662A |
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Sample: Tissue homogenate |
DOI: 10.1111/jphp.13016 |
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Sample: Tissue homogenate |
H************aSubmitted [ Nov 21 2022 ]
Asked: Can we measure the URL for Rat cTn-I/TNNI3 (Cardiac Troponin I) CLIA Kit with spectrophotometer instead of Chemiluminescence immunoassay analyzer?
Reply
adminSubmitted [ Nov 21 2022 ]
Answered: Hi, It is not recommended to use spectrophotometer instead of chemiluminescence immunoassay analyzer. The detection principles of these two instruments are different. If you need to use a spectrophotometer or a microplate reader, we suggest you choose the following products. E-EL-R1253 Rat TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit https://www.elabscience.com/p-rat_tnni3_ctn_i_troponin_i_type_3_cardiac_elisa_kit-22328.html Best regards