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Rat cTn-I/TNNI3 (Cardiac Troponin I) CLIA Kit

Cat.No.:E-CL-R0721
Reactivity: Rat

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Product Details

Properties

Assay type	Sandwich-CLIA
Format	96T/48T
Assay time	3.5h
Detection range	12.50-800 pg/mL
Sensitivity	7.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat cTn-I/TNNI3 in samples. No significant cross-reactivity or interference between Rat cTn-I/TNNI3 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 15%.
Application	This CLIA kit applies to the in vitro quantitative determination of Rat cTn-I/TNNI3 concentrations in serum, plasma and other biological fluids.

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat cTn-I/TNNI3. Standards or samples are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat cTn-I/TNNI3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat cTn-I/TNNI3, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of Rat cTn-I/TNNI3. You can calculate the concentration of Rat cTn-I/TNNI3 in the samples by comparing the RLU value of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro CLIA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent A	1 vial, 5 mL	2-8℃(Protect from light)
Substrate Reagent B	1 vial, 5 mL	2-8℃(Protect from light)
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat cTn-I/TNNI3 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat cTn-I/TNNI3 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	43.14	127.67	380.90	42.34	115.95	403.72
Standard deviation	4.31	14.45	34.93	5.40	10.41	45.38
CV (%)	9.99	11.32	9.17	12.75	8.98	11.24

Recovery

The recovery of Rat cTn-I/TNNI3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-103	95
EDTA plasma(n=8)	95-109	103
Cell culture media(n=8)	89-102	96

Linearity

Samples were spiked with high concentrations of Rat cTn-I/TNNI3 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	TNNC1, cTnI, CMD1FF, CMD2A, CMH7, RCM1
Research Area	Cancer, Cardiovascular, Developmental biology, Signal transduction, Stem cells

Assay Procedures

	1.Add 100 μL of standard or sample to each well. Incubate for 90 min at 37℃.
	2.Remove the liquid.
	3.Add 100 μL Biotinylated Detection Ab. Incubate 1 hour at 37℃. Aspirate and wash 3 times.
	4.Add 100 μL HRP Conjugate. Incubate 30 min at 37℃. Aspirate and wash 5 times.
	5.Add 100 μL Substrate Mixture Solution. Incubate for 5 min at 37℃.
	6.Fluorescence appeared. Measure the RLU value with the Chemiluminescence immunoassay analyzer. Calculation of the results.

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

Journal of the American Heart Association (2022) IF: 5.501

Trimetazidine Alleviates Postresuscitation Myocardial Dysfunction and Improves 96‐Hour Survival in a Ventricular Fibrillation Rat Model

DOI: 10.1161/JAHA.121.023378
	Sample: plasma

RSC Advances (2019) IF: 3.049

Morroniside alleviates coxsackievirus B3-induced myocardial damage apoptosis via restraining NLRP3 inflammasome activation

DOI: 10.1039/C8RA08662A
	Sample: Tissue homogenate

JOURNAL OF PHARMACY AND PHARMACOLOGY (2018) IF: 2.309

Total flavones of Rhododendron simsii Planch flower protect isolated rat heart from ischaemia‐reperfusion injury and its mechanism of UTR‐RhoA‐ROCK pathway inhibition

DOI: 10.1111/jphp.13016
	Sample: Tissue homogenate

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Reviews/Q&A

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Reviews
Q&A

H************aSubmitted [ Nov 21 2022 ]

Asked: Can we measure the URL for Rat cTn-I/TNNI3 (Cardiac Troponin I) CLIA Kit with spectrophotometer instead of Chemiluminescence immunoassay analyzer?

adminSubmitted [ Nov 21 2022 ]

Answered: Hi, It is not recommended to use spectrophotometer instead of chemiluminescence immunoassay analyzer. The detection principles of these two instruments are different. If you need to use a spectrophotometer or a microplate reader, we suggest you choose the following products. E-EL-R1253 Rat TNNI3/cTn-I(Troponin I Type 3, Cardiac) ELISA Kit https://www.elabscience.com/p-rat_tnni3_ctn_i_troponin_i_type_3_cardiac_elisa_kit-22328.html Best regards

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