Rat CXCR3 (CXC-Chemokine Receptor 3) CLIA Kit (E-CL-R0190)

Name: Rat CXCR3 (CXC-Chemokine Receptor 3) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0190
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat CXCR3 (CXC-Chemokine Receptor 3) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat CXCR3 in samples.No significant cross-reactivity or interference between Rat CXCR3 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	55706.000	58882.000	57294.000	57260
1000	23928.000	28666.000	26297.000	26263
500	12471.000	10979.000	11725.000	11691
250	5208.000	6194.000	5701.000	5667
125	2899.000	2473.000	2686.000	2652
62.5	1472.000	1346.000	1409.000	1375
31.25	733.000	745.000	739.000	705
0	34.000	34.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	95.080	278.500	930.300	102.100	277.960	944.410
Standard deviation	10.080	21.890	84.290	11.610	29.440	103.410
CV（%）	10.600	7.860	9.060	11.370	10.590	10.950

Recovery

The recovery of Rat CXCR3 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-102	97
EDTA plasma （n=8）	87-98	92
Cell culture media （n=8）	95-112	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat CXCR3 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	93-108	97-110	87-102
1:2	Average	99	102	94
1:4	Range	84-97	92-103	94-108
1:4	Average	91	97	101
1:8	Range	98-113	100-118	103-118
1:8	Average	106	107	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat CXCR3. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat CXCR3 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat CXCR3, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat CXCR3. You can calculate the concentration of Rat CXCR3 in the samples by comparing the RLU value of the samples to the standard curve.

Background