Rat DRD2 (Dopamine Receptor D2) CLIA Kit (E-CL-R0220)

Name: Rat DRD2 (Dopamine Receptor D2) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0220
Price: 180.0 USD
Availability: InStock

Copy the product info.

Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat DRD2 (Dopamine Receptor D2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

Bulk request

Add to cart

For research use only. Order now, ship in 3 days

Product Summary

Sensitivity	0.19 mIU/mL
Detection Range	0.31-20 mIU/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat DRD2 in samples.No significant cross-reactivity or interference between Rat DRD2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

mIU/mL	OD1	OD2	Mean OD	Corrected OD
20	34051.000	38951.000	36501.000	36468
10	13299.000	13615.000	13457.000	13424
5	5716.000	4832.000	5274.000	5241
2.5	2537.000	2879.000	2708.000	2675
1.25	1512.000	1488.000	1500.000	1467
0.63	981.000	915.000	948.000	915
0.31	648.000	680.000	664.000	631
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（mIU/mL）	1.050	2.580	7.970	0.980	2.710	7.810
Standard deviation	0.100	0.280	0.550	0.120	0.200	0.790
CV（%）	9.520	10.850	6.900	12.240	7.380	10.120

Recovery

The recovery of Rat DRD2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-100	93
EDTA plasma （n=8）	84-98	90
Cell culture media （n=8）	96-109	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat DRD2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	84-100	94-110	98-113
1:2	Average	91	100	106
1:4	Range	85-100	103-115	98-113
1:4	Average	91	109	105
1:8	Range	101-117	90-106	100-113
1:8	Average	109	97	107

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DRD2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DRD2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DRD2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat DRD2. You can calculate the concentration of Rat DRD2 in the samples by comparing the RLU value of the samples to the standard curve.

Background