Rat DRD4 (Dopamine Receptor D4) CLIA Kit (E-CL-R0222)

Name: Rat DRD4 (Dopamine Receptor D4) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0222
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat DRD4 (Dopamine Receptor D4) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat DRD4 in samples.No significant cross-reactivity or interference between Rat DRD4 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	56354.000	59100.000	57727.000	57697
1000	23568.000	27558.000	25563.000	25533
500	12390.000	11018.000	11704.000	11674
250	4885.000	5851.000	5368.000	5338
125	2810.000	2604.000	2707.000	2677
62.5	1445.000	1387.000	1416.000	1386
31.25	700.000	704.000	702.000	672
0	29.000	31.000	30.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	94.730	214.580	774.080	98.170	195.370	839.440
Standard deviation	7.930	17.400	77.490	12.560	19.710	88.560
CV（%）	8.370	8.110	10.010	12.790	10.090	10.550

Recovery

The recovery of Rat DRD4 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	100-114	107
EDTA plasma （n=8）	94-108	101
Cell culture media （n=8）	87-100	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat DRD4 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-107	96-106	91-103
1:2	Average	98	101	97
1:4	Range	95-110	102-115	87-99
1:4	Average	100	109	92
1:8	Range	103-116	89-104	95-107
1:8	Average	109	96	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat DRD4. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat DRD4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat DRD4, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat DRD4. You can calculate the concentration of Rat DRD4 in the samples by comparing the RLU value of the samples to the standard curve.

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