Rat GAL7 (Galectin 7) CLIA Kit (E-CL-R0265)

Name: Rat GAL7 (Galectin 7) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0265
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat GAL7 in samples.No significant cross-reactivity or interference between Rat GAL7 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	54256.000	63398.000	58827.000	58798
1000	22645.000	27115.000	24880.000	24851
500	13033.000	10947.000	11990.000	11961
250	5670.000	5828.000	5749.000	5720
125	2809.000	2797.000	2803.000	2774
62.5	1463.000	1339.000	1401.000	1372
31.25	670.000	754.000	712.000	683
0	28.000	30.000	29.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	91.490	316.640	685.930	93.110	335.810	631.040
Standard deviation	10.480	22.800	50.070	10.820	34.690	68.220
CV（%）	11.450	7.200	7.300	11.620	10.330	10.810

Recovery

The recovery of Rat GAL7 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	87-102	93
EDTA plasma （n=8）	96-108	101
Cell culture media （n=8）	99-113	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat GAL7 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	98-113	87-100	98-111
1:2	Average	106	94	104
1:4	Range	97-108	87-99	102-119
1:4	Average	102	93	109
1:8	Range	103-118	97-111	100-117
1:8	Average	109	104	108

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GAL7. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GAL7 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GAL7, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GAL7. You can calculate the concentration of Rat GAL7 in the samples by comparing the RLU value of the samples to the standard curve.

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