Rat GDH/GLDH(Glutamate Dehydrogenase) CLIA Kit (E-CL-R0292)

Name: Rat GDH/GLDH(Glutamate Dehydrogenase) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0292
Price: 180.0 USD
Availability: InStock

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Rat GDH/GLDH(Glutamate Dehydrogenase) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	75 pg/mL
Detection Range	125-8000 pg/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat GDH/GLDH in samples.No significant cross-reactivity or interference between Rat GDH/GLDH and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
8000	70701.000	75245.000	72973.000	72941
4000	34575.000	39153.000	36864.000	36832
2000	19806.000	18224.000	19015.000	18983
1000	8506.000	8984.000	8745.000	8713
500	4233.000	4029.000	4131.000	4099
250	1878.000	1868.000	1873.000	1841
125	736.000	832.000	784.000	752
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	392.110	1092.380	2805.110	402.470	1092.840	2759.340
Standard deviation	48.270	91.650	202.530	34.010	78.680	251.650
CV（%）	12.310	8.390	7.220	8.450	7.200	9.120

Recovery

The recovery of Rat GDH/GLDH was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	90-104	96
EDTA plasma （n=8）	97-108	102
Cell culture media （n=8）	103-114	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat GDH/GLDH and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-105	91-104	100-113
1:2	Average	96	97	107
1:4	Range	98-114	88-101	89-104
1:4	Average	104	94	96
1:8	Range	92-103	101-118	103-118
1:8	Average	97	110	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GDH/GLDH. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GDH/GLDH and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GDH/GLDH, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GDH/GLDH. You can calculate the concentration of Rat GDH/GLDH in the samples by comparing the RLU value of the samples to the standard curve.