Rat GLβ (Galactosidase, Beta) CLIA Kit (E-CL-R0258)

Name: Rat GLβ (Galactosidase, Beta) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0258
Price: 180.0 USD
Availability: InStock

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Rat GLβ (Galactosidase, Beta) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat GLβ in samples.No significant cross-reactivity or interference between Rat GLβ and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	58543.000	59829.000	59186.000	59160
1000	24552.000	28132.000	26342.000	26316
500	11656.000	11164.000	11410.000	11384
250	5654.000	5942.000	5798.000	5772
125	2928.000	2578.000	2753.000	2727
62.5	1435.000	1239.000	1337.000	1311
31.25	646.000	728.000	687.000	661
0	25.000	27.000	26.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	93.840	254.470	811.240	89.370	256.680	789.770
Standard deviation	10.050	29.930	70.900	11.150	27.230	89.170
CV（%）	10.710	11.760	8.740	12.480	10.610	11.290

Recovery

The recovery of Rat GLβ was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	92-109	99
EDTA plasma （n=8）	88-99	93
Cell culture media （n=8）	92-108	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat GLβ and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-101	86-98	101-117
1:2	Average	96	92	107
1:4	Range	84-96	96-109	95-111
1:4	Average	90	101	102
1:8	Range	95-108	99-116	89-103
1:8	Average	101	106	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GLβ. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GLβ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GLβ, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GLβ. You can calculate the concentration of Rat GLβ in the samples by comparing the RLU value of the samples to the standard curve.

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