Rat GREM1 (GremLin 1) CLIA Kit (E-CL-R0309)

Name: Rat GREM1 (GremLin 1) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0309
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat GREM1 in samples.No significant cross-reactivity or interference between Rat GREM1 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	53565.000	60575.000	57070.000	57031
1000	22576.000	26020.000	24298.000	24259
500	12009.000	11193.000	11601.000	11562
250	5679.000	5739.000	5709.000	5670
125	2776.000	2534.000	2655.000	2616
62.5	1352.000	1302.000	1327.000	1288
31.25	684.000	764.000	724.000	685
0	38.000	40.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	108.210	304.790	839.310	116.850	294.210	776.230
Standard deviation	12.640	21.670	66.730	15.180	32.690	54.490
CV（%）	11.680	7.110	7.950	12.990	11.110	7.020

Recovery

The recovery of Rat GREM1 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	97-111	103
EDTA plasma （n=8）	90-105	97
Cell culture media （n=8）	100-115	106

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat GREM1 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-105	91-104	88-104
1:2	Average	99	97	96
1:4	Range	92-107	100-116	93-107
1:4	Average	98	107	99
1:8	Range	94-108	90-101	90-103
1:8	Average	99	96	95

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat GREM1. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat GREM1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat GREM1, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat GREM1. You can calculate the concentration of Rat GREM1 in the samples by comparing the RLU value of the samples to the standard curve.

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