Rat HSP-40 (Heat Shock Protein 40) CLIA Kit (E-CL-R0324)

Name: Rat HSP-40 (Heat Shock Protein 40) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0324
Price: 180.0 USD
Availability: InStock

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Rat HSP-40 (Heat Shock Protein 40) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat HSP-40 in samples.No significant cross-reactivity or interference between Rat HSP-40 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	62332.000	64486.000	63409.000	63377
2000	28091.000	32965.000	30528.000	30496
1000	15375.000	13305.000	14340.000	14308
500	6589.000	7833.000	7211.000	7179
250	3543.000	3205.000	3374.000	3342
125	1633.000	1613.000	1623.000	1591
62.5	759.000	789.000	774.000	742
0	31.000	33.000	32.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	182.610	350.480	1622.510	195.920	360.470	1585.440
Standard deviation	17.000	39.150	163.710	22.410	32.330	129.530
CV（%）	9.310	11.170	10.090	11.440	8.970	8.170

Recovery

The recovery of Rat HSP-40 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	101-117	107
EDTA plasma （n=8）	94-110	101
Cell culture media （n=8）	94-109	102

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat HSP-40 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-101	103-116	97-113
1:2	Average	94	110	104
1:4	Range	101-114	85-98	97-113
1:4	Average	106	91	103
1:8	Range	85-100	88-104	102-116
1:8	Average	92	95	109

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat HSP-40. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat HSP-40 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat HSP-40, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat HSP-40. You can calculate the concentration of Rat HSP-40 in the samples by comparing the RLU value of the samples to the standard curve.

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