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Rat ICAM-1/CD54(Intercellular Adhesion Molecule 1) ELISA Kit

  • Cat.No.:E-EL-R2850

  • Reactivity: Rat

To Purchase E-EL-R2850

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $609
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat ICAM-1/CD54 in samples. No significant cross-reactivity or interference between Rat ICAM-1/CD54 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat ICAM-1/CD54 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat ICAM-1/CD54. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat ICAM-1/CD54 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat ICAM-1/CD54, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat ICAM-1/CD54. You can calculate the concentration of Rat ICAM-1/CD54 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat ICAM-1/CD54 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat ICAM-1/CD54 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 2.21 5.80 12.50 2.10 5.80 12.50
Standard deviation 0.10 0.23 0.62 0.06 0.41 0.75
CV (%) 4.32 5.30 4.20 3.10 5.34 6.30

Recovery

The recovery of Rat ICAM-1/CD54 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 86-98 92
EDTA plasma (n=8) 95-109 101
Cell culture media (n=8) 89-104 96

Linearity

Samples were spiked with high concentrations of Rat ICAM-1/CD54 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsBB2, P3.58
Research AreaCancer, Cardiovascular, Immunology, Stem cells

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. BIOMACROMOLECULES (2021) IF: 6.988
    Fabrication of In Situ Layered Hydrogel Scaffold for the Co-delivery of PGDF-BB/Chlorhexidine to Regulate Proinflammatory Cytokines, Growth Factors, and MMP-9 in a Diabetic Skin Defect Albino Rat Model

    DOI: 10.1021/acs.biomac.0c01709

    PMID: 33899465

    Sample: Tissue homogenate,Serum
  2. Scientific Reports (2016) IF: 5.228
    Endothelial dysfunction correlates with decompression bubbles in rats

    DOI: 10.1038/srep33390

    Sample: Serum
  3. JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546
    Autophagy decreases alveolar epithelial cell injury by regulating the release of inflammatory mediators

    DOI: 10.1002/jcp.29453

    PMID: 31960959

    Sample: Cell culture supernatant,Tissue homogenate
  4. RESPIRATORY RESEARCH (2021) IF: 5.631
    Artesunate ameliorates cigarette smoke-induced airway remodelling via PPAR-γ/TGF-β1/Smad2/3 signalling pathway

    DOI: 10.1186/s12931-021-01687-y

    PMID: 33757521

    Sample: BALF
  5. EXPERIMENTAL BIOLOGY AND MEDICINE (2020) IF: 3.139
    H2S attenuates oxidative stress via Nrf2/NF-κB signaling to regulate restenosis after percutaneous transluminal angioplasty:

    DOI: 10.1177/1535370220961038

    Sample: Plasma
  6. PHARMACEUTICAL BIOLOGY (2021) IF: 3.503
    Gynura procumbens ethanol extract improves vascular dysfunction by suppressing inflammation in postmenopausal rats fed a high-fat diet

    DOI: 10.1080/13880209.2021.1970199

    PMID: 34493166

    Sample: aortic tissue
  7. PHARMACEUTICAL BIOLOGY (2022) IF: 3.503
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    Sample: serum
  8. CLINICAL HEMORHEOLOGY AND MICROCIRCULATION (2020) IF: 2.375
    Chronic intermittent hypoxia vs chronic continuous hypoxia: Effects on vascular endothelial function and myocardial contractility

    DOI: 10.3233/CH-190706

    Sample: serum
  9. Brazilian Journal of Anesthesiology (2021)
    Dose-related effects of dexmedetomidine on sepsis-initiated lung injury in rats

    DOI: 10.1016/j.bjane.2021.02.051

    Sample: plasma
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