Rat IFN-γ (Interferon Gamma) CLIA Kit (E-CL-R0009)

Name: Rat IFN-γ (Interferon Gamma) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0009
Price: 180.0 USD
Availability: InStock

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Rat IFN-γ (Interferon Gamma) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat IFN-γ in samples.No significant cross-reactivity or interference between Rat IFN-γ and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	55362.000	58810.000	57086.000	57058
400	22391.000	24885.000	23638.000	23610
200	10075.000	9673.000	9874.000	9846
100	4633.000	4859.000	4746.000	4718
50	2287.000	2105.000	2196.000	2168
25	1161.000	1121.000	1141.000	1113
12.5	505.000	551.000	528.000	500
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	41.290	111.110	379.650	39.600	105.390	391.620
Standard deviation	3.660	12.860	29.880	4.940	10.750	30.620
CV（%）	8.860	11.570	7.870	12.470	10.200	7.820

Recovery

The recovery of Rat IFN-γ was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-106	99
EDTA plasma （n=8）	85-98	92
Cell culture media （n=8）	88-101	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat IFN-γ and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	90-105	100-112	88-102
1:2	Average	98	106	93
1:4	Range	89-101	90-102	85-97
1:4	Average	94	95	91
1:8	Range	101-116	101-113	86-99
1:8	Average	109	107	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IFN-γ. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IFN-γ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IFN-γ, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat IFN-γ. You can calculate the concentration of Rat IFN-γ in the samples by comparing the RLU value of the samples to the standard curve.

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