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Rat IgA(Immunoglobulin A) ELISA Kit

Citations(5)

Cat.No.:E-EL-R3015
Reactivity: Rat

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T/24
Assay time	3.5h
Detection range	7.81-500 pg/mL
Sensitivity	4.69 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat IgA in samples. No significant cross-reactivity or interference between Rat IgA and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat IgA concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgA. You can calculate the concentration of Rat IgA in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 12 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 12 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 12 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IgA were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IgA were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	21.90	57.79	227.30	23.00	58.80	239.04
Standard deviation	1.21	2.73	11.02	1.16	2.83	10.69
CV (%)	5.53	4.72	4.85	5.04	4.81	4.47

Recovery

The recovery of Rat IgA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	92-104	97
EDTA plasma (n=8)	100-109	103
Cell culture media (n=8)	84-98	90

Linearity

Samples were spiked with high concentrations of Rat IgA and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	IgA
Research Area	Cancer, Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Frontiers in Bioengineering and Biotechnology (2022) IF: 6.064

Safety and biodistribution of exosomes derived from human induced pluripotent stem cells.

DOI: 10.3389/fbioe.2022.949724
	Sample: Blood

Journal of Zhejiang University-SCIENCE B (2019) IF: 1.879

Improvement in post-partum uterine involution in rats treated with Apios americana

DOI: 10.1631/jzus.B1800475
	Sample: Serum

JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2018) IF: 3.755

Comparison of the effect of dietary copper nanoparticles and one copper (II) salt on the metabolic and immune status in a rat model

DOI: 10.1016/j.jtemb.2018.03.017
	Sample: Plasma

FASEB JOURNAL (2018) IF: 5.595

A novel immunodeficient rat model supports human lung cancer xenografts

DOI: 10.1096/fj.201800102RR
	Sample: Serum

BRITISH JOURNAL OF PHARMACOLOGY (2018) IF: 6.81

Alcohol binge disrupts the rat intestinal barrier: the partial protective role of oleoylethanolamide

DOI: 10.1111/bph.14501
PMID: 30248186	Sample: Plasma,Tissue Homogenate

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