Click here to view cell apoptosis assay products

Rat IgE(Immunoglobulin E) ELISA Kit

  • Cat.No.:E-EL-R0517

  • Reactivity: Rat

To Purchase E-EL-R0517

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 1.56-100 ng/mL
Sensitivity 0.94 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat IgE in samples. No significant cross-reactivity or interference between Rat IgE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat IgE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgE. You can calculate the concentration of Rat IgE in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IgE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IgE were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 4.80 10.30 45.80 5.30 10.80 47.50
Standard deviation 0.20 0.50 2.30 0.30 0.50 2.40
CV (%) 4.17 4.85 5.02 5.66 4.63 5.05


The recovery of Rat IgE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-102 96
EDTA plasma (n=8) 90-103 96
Cell culture media (n=8) 91-104 98


Samples were spiked with high concentrations of Rat IgE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Research AreaImmunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. MOLECULAR IMMUNOLOGY (2017) IF: 3.236
    The protective effect of dexmedetomidine on LPS-induced acute lung injury through the HMGB1-mediated TLR4/NF-κB and PI3K/Akt/mTOR pathways

    DOI: 10.1016/j.molimm.2017.12.008

    Sample: Bronchoalveolar Lavage Fluid,Serum,Tissue homogena
    Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma

    DOI: 10.1016/j.biopha.2019.109188

    PMID: 31315072

    Sample: Serum,Tissue homogenate
    Comparison of the effect of dietary copper nanoparticles and one copper (II) salt on the metabolic and immune status in a rat model

    DOI: 10.1016/j.jtemb.2018.03.017

    Sample: plasma
  4. Genes (2020) IF: 3.759
    Allergic Inflammation Alters microRNA Expression Profile in Adipose Tissue in the Rat

    DOI: 10.3390/genes11091034

    PMID: 32887419

    Sample: Serum
    Zinc and iron complexes of oleanolic acid, (OA) attenuate allergic airway inflammation in rats

    DOI: 10.1007/s10787-019-00597-2

    Sample: serum
  6. Molecular Medicine Reports (2019) IF: 2.952
    Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats

    DOI: 10.3892/mmr.2019.10694

    Sample: serum
  7. Analytical Methods (2015) IF: 1.821
    The metabonomic study of Shaoyao-Gancao decoction in a rat model of acute bronchial asthma by 1H NMR

    DOI: 10.1039/C5AY01701G

    Sample: Serum
  8. International Journal of Clinical and Experimental Pathology (2015) IF: 1.891
    Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation

    PMID: 26722390

    Sample: bronchoalveolar lavage fluid (BALF)
Show AllShorten


  • Show all
  • Reviews
  • Q&A
... Show All Show Less

People Also Bought

Apply for Free Shipping 24T ELISA Kit
*Product Name:
*Catalog Number:
*When will you use it?