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Rat IgE(Immunoglobulin E) ELISA Kit

Cat.No.:E-EL-R0517
Reactivity: Rat

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	1.56-100 ng/mL
Sensitivity	0.94 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat IgE in samples. No significant cross-reactivity or interference between Rat IgE and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat IgE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat IgE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat IgE. You can calculate the concentration of Rat IgE in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat IgE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat IgE were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	4.80	10.30	45.80	5.30	10.80	47.50
Standard deviation	0.20	0.50	2.30	0.30	0.50	2.40
CV (%)	4.17	4.85	5.02	5.66	4.63	5.05

Recovery

The recovery of Rat IgE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-102	96
EDTA plasma (n=8)	90-103	96
Cell culture media (n=8)	91-104	98

Linearity

Samples were spiked with high concentrations of Rat IgE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	IgE
Research Area	Immunology

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

MOLECULAR IMMUNOLOGY (2017) IF: 3.236
The protective effect of dexmedetomidine on LPS-induced acute lung injury through the HMGB1-mediated TLR4/NF-κB and PI3K/Akt/mTOR pathways

DOI: 10.1016/j.molimm.2017.12.008

Sample: Bronchoalveolar Lavage Fluid,Serum,Tissue homogena
BIOMEDICINE & PHARMACOTHERAPY (2019) IF: 3.743
Suhuang antitussive capsule inhibits NLRP3 inflammasome activation and ameliorates pulmonary dysfunction via suppression of endoplasmic reticulum stress in cough variant asthma

DOI: 10.1016/j.biopha.2019.109188

PMID: 31315072
Sample: Serum,Tissue homogenate
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2018) IF: 3.755
Comparison of the effect of dietary copper nanoparticles and one copper (II) salt on the metabolic and immune status in a rat model

DOI: 10.1016/j.jtemb.2018.03.017

Sample: plasma
Genes (2020) IF: 3.759
Allergic Inflammation Alters microRNA Expression Profile in Adipose Tissue in the Rat

DOI: 10.3390/genes11091034

PMID: 32887419
Sample: Serum
INFLAMMOPHARMACOLOGY (2019) IF: 3.838
Zinc and iron complexes of oleanolic acid, (OA) attenuate allergic airway inflammation in rats

DOI: 10.1007/s10787-019-00597-2

Sample: serum
Molecular Medicine Reports (2019) IF: 2.952
Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats

DOI: 10.3892/mmr.2019.10694

Sample: serum
Analytical Methods (2015) IF: 1.821
The metabonomic study of Shaoyao-Gancao decoction in a rat model of acute bronchial asthma by 1H NMR

DOI: 10.1039/C5AY01701G

Sample: Serum
International Journal of Clinical and Experimental Pathology (2015) IF: 1.891
Downregulation of SUMF2 gene in ovalbumin-induced rat model of allergic inflammation

PMID: 26722390
Sample: bronchoalveolar lavage fluid (BALF)

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