Rat IgG (Immunoglobulin G) CLIA Kit (E-CL-R0356)

Name: Rat IgG (Immunoglobulin G) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0356
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat IgG in samples.No significant cross-reactivity or interference between Rat IgG and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	49017.000	54521.000	51769.000	51730
250	20929.000	23665.000	22297.000	22258
125	9823.000	9105.000	9464.000	9425
62.5	4621.000	4791.000	4706.000	4667
31.25	2636.000	2276.000	2456.000	2417
15.63	1357.000	1251.000	1304.000	1265
7.81	786.000	840.000	813.000	774
0	38.000	40.000	39.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	25.050	67.760	201.640	27.270	66.040	207.230
Standard deviation	2.520	4.960	17.780	3.190	7.250	15.500
CV（%）	10.060	7.320	8.820	11.700	10.980	7.480

Recovery

The recovery of Rat IgG was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-106	98
EDTA plasma （n=8）	91-106	98
Cell culture media （n=8）	87-102	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat IgG and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-110	99-115	88-101
1:2	Average	104	107	95
1:4	Range	89-104	96-111	85-96
1:4	Average	95	102	91
1:8	Range	98-112	93-106	94-108
1:8	Average	105	98	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IgG. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IgG and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IgG, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat IgG. You can calculate the concentration of Rat IgG in the samples by comparing the RLU value of the samples to the standard curve.

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