Rat IL-1β (Interleukin 1 Beta) CLIA Kit
Price: $ 682
Price: $ 546
Price: $ 180
Price: Inquire
Price: Inquire
Size:
96T
48T
24T
96T*10
96T*5
Quantity:
- Reactivity: Rat
- Detection Range: 12.5-800 pg/mL
- Sensitivity: 7.5 pg/mL
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Product Details
Test Principle | This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat IL-1β. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat IL-1β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat IL-1β, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat IL-1β. You can calculate the concentration of Rat IL-1β in the samples by comparing the RLU value of the samples to the standard curve. |
Assay Type | Sandwich-ELISA |
size | 96T / 48T / 24T / 96T*10 / 96T*5 |
Assay Time | 3.5h |
Detection Method | Chemiluminescence |
Detection Range | 12.5-800 pg/mL |
Sensitivity | 7.5 pg/mL |
Sample Volume | 100μL |
Sample Type | serum, plasma and other biological fluids |
Specificity | This kit recognizes Rat IL-1β in samples.No significant cross-reactivity or interference between Rat IL-1β and analogues was observed |
Precision | Both intra-CV and inter-CV are < 10%. |
Recovery | 80%-120% |
Introduction | This ELISA kit applies to the in vitro quantitative determination of Rat IL-1β concentrations in serum, plasma and other biological fluids. |
Applications | CLIA |
Storage | 2-8℃/-20℃ |
Target Information
Database | SwissProt: Q63264 |
Syonoyms | IL1B,IL1-BETA,IL1F2,catabolin |
Research Area | Cancer , Cardiovascular , Metabolism , Immunology |
Assay Procedures
Citations
-
Neuroprotective efficacy of 4-Hydroxyisoleucine in experimentally induced intracerebral hemorrhage
IF:4.219
Journal:SAUDI JOURNAL OF BIOLOGICAL SCIENCES
DOI:10.1016/j.sjbs.2021.07.010
PMID:34764759
-
the NF-κB Pathway in Rats
IF:4.092
Journal:INFLAMMATION