Rat INSR-β (Insulin Receptor Beta Subunit) CLIA Kit (E-CL-R0366)

Name: Rat INSR-β (Insulin Receptor Beta Subunit) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0366
Price: 180.0 USD
Availability: InStock

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Citations ({{ citationsPage.numTotal }}) Manual MSDS

Rat INSR-β (Insulin Receptor Beta Subunit) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.09 ng/mL
Detection Range	0.16-10 ng/mL
Sample Volume	100 μL
Total Assay Time	4 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat INSR-β in samples.No significant cross-reactivity or interference between Rat INSR-β and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

ng/mL	OD1	OD2	Mean OD	Corrected OD
10	34601.000	36301.000	35451.000	35416
5	15820.000	16514.000	16167.000	16132
2.5	8148.000	7612.000	7880.000	7845
1.25	3829.000	3961.000	3895.000	3860
0.63	1874.000	1762.000	1818.000	1783
0.31	918.000	864.000	891.000	856
0.16	374.000	408.000	391.000	356
0	33.000	37.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（ng/mL）	0.510	0.950	4.760	0.500	0.870	4.580
Standard deviation	0.060	0.070	0.530	0.050	0.080	0.420
CV（%）	11.760	7.370	11.130	10.000	9.200	9.170

Recovery

The recovery of Rat INSR-β was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-103	96
EDTA plasma （n=8）	92-107	99
Cell culture media （n=8）	102-114	108

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat INSR-β and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	99-111	99-113	97-108
1:2	Average	105	107	103
1:4	Range	87-99	97-111	87-103
1:4	Average	92	103	94
1:8	Range	88-100	95-109	95-112
1:8	Average	95	102	103

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat INSR-β. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat INSR-β and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat INSR-β, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat INSR-β. You can calculate the concentration of Rat INSR-β in the samples by comparing the RLU value of the samples to the standard curve.