Rat LEP (Leptin) CLIA Kit (E-CL-R0416)

Name: Rat LEP (Leptin) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0416
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	18.75 pg/mL
Detection Range	31.25-2000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat LEP in samples.No significant cross-reactivity or interference between Rat LEP and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
2000	52125.000	59933.000	56029.000	55994
1000	25485.000	26383.000	25934.000	25899
500	11711.000	10809.000	11260.000	11225
250	5042.000	5826.000	5434.000	5399
125	2686.000	2552.000	2619.000	2584
62.5	1383.000	1309.000	1346.000	1311
31.25	668.000	738.000	703.000	668
0	34.000	36.000	35.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	99.970	293.390	865.850	101.830	275.260	800.740
Standard deviation	9.560	31.570	65.890	10.440	27.170	89.520
CV（%）	9.560	10.760	7.610	10.250	9.870	11.180

Recovery

The recovery of Rat LEP was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-107	102
EDTA plasma （n=8）	94-107	100
Cell culture media （n=8）	92-108	99

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat LEP and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	85-98	95-108	89-104
1:2	Average	90	101	96
1:4	Range	88-101	88-100	88-99
1:4	Average	93	93	94
1:8	Range	87-100	90-103	86-98
1:8	Average	92	97	93

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat LEP. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat LEP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat LEP, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat LEP. You can calculate the concentration of Rat LEP in the samples by comparing the RLU value of the samples to the standard curve.

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