Rat MAPτ (Microtubule Associated Protein Tau) CLIA Kit (E-CL-R0629)

Name: Rat MAPτ (Microtubule Associated Protein Tau) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0629
Price: 180.0 USD
Availability: InStock

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Rat MAPτ (Microtubule Associated Protein Tau) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	7.5 pg/mL
Detection Range	12.5-800 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat MAPτ in samples.No significant cross-reactivity or interference between Rat MAPτ and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
800	53433.000	59803.000	56618.000	56590
400	21684.000	25636.000	23660.000	23632
200	10458.000	9988.000	10223.000	10195
100	4857.000	5051.000	4954.000	4926
50	2326.000	2076.000	2201.000	2173
25	1194.000	1028.000	1111.000	1083
12.5	519.000	561.000	540.000	512
0	28.000	28.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	42.380	79.950	285.190	38.860	84.510	262.950
Standard deviation	4.640	8.700	29.350	3.920	6.350	29.420
CV（%）	10.950	10.880	10.290	10.090	7.510	11.190

Recovery

The recovery of Rat MAPτ was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	93-105	99
EDTA plasma （n=8）	92-108	99
Cell culture media （n=8）	101-113	107

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat MAPτ and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-108	90-107	86-101
1:2	Average	102	97	92
1:4	Range	89-103	99-114	101-117
1:4	Average	97	107	106
1:8	Range	86-100	92-104	99-114
1:8	Average	91	98	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MAPτ. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAPτ and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAPτ, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat MAPτ. You can calculate the concentration of Rat MAPτ in the samples by comparing the RLU value of the samples to the standard curve.

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