Rat MAU (Microalbuminuria) CLIA Kit (E-CL-R0023)

Name: Rat MAU (Microalbuminuria) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0023
Price: 180.0 USD
Availability: InStock

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Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	0.19 μg/mL
Detection Range	0.31-20 μg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat MAU in samples.No significant cross-reactivity or interference between Rat MAU and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

μg/mL	OD1	OD2	Mean OD	Corrected OD
20	34889.000	38511.000	36700.000	36667
10	11909.000	14049.000	12979.000	12946
5	5563.000	5423.000	5493.000	5460
2.5	2610.000	2632.000	2621.000	2588
1.25	1520.000	1418.000	1469.000	1436
0.63	905.000	859.000	882.000	849
0.31	654.000	670.000	662.000	629
0	32.000	34.000	33.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（μg/mL）	1.060	2.460	7.190	1.080	2.480	7.210
Standard deviation	0.110	0.180	0.590	0.100	0.200	0.550
CV（%）	10.380	7.320	8.210	9.260	8.060	7.630

Recovery

The recovery of Rat MAU was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-107	98
EDTA plasma （n=8）	98-111	105
Cell culture media （n=8）	88-102	94

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat MAU and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	89-100	101-120	94-106
1:2	Average	94	109	100
1:4	Range	93-107	95-108	87-101
1:4	Average	99	102	92
1:8	Range	94-108	89-102	96-111
1:8	Average	101	96	101

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat MAU. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MAU and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MAU, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat MAU. You can calculate the concentration of Rat MAU in the samples by comparing the RLU value of the samples to the standard curve.

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