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Rat MCP-1(Monocyte Chemotactic Protein 1) ELISA Kit

Citations(29)

  • Cat.No.:E-EL-R0633

  • Reactivity: Rat
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  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
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Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat MCP-1 in samples. No significant cross-reactivity or interference between Rat MCP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat MCP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MCP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MCP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MCP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MCP-1. You can calculate the concentration of Rat MCP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips 		-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL 		-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MCP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MCP-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.10 4.40 0.50 1.20 4.70
Standard deviation 0.03 0.05 0.15 0.03 0.07 0.17
CV (%) 6.00 4.55 3.41 6.00 5.83 3.62

Recovery

The recovery of Rat MCP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-109 100
EDTA plasma (n=8) 97-111 102
Cell culture media (n=8) 86-101 93

Linearity

Samples were spiked with high concentrations of Rat MCP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsCCL2, GDCF-2, HC11, HSMCR30, MCAF, MCP1, SCYA2, SMC-CF
Research AreaCancer, Cardiovascular, Metabolism, Immunology

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
elisa assay procedure 3

3. Aspirate and wash the plate for 3 times
elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
elisa assay procedure 6

6. Add 50μL Stop Solution
elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

  1. Advanced Fiber Materials (2022) IF: 12.958
    Rock Climbing-Inspired Electrohydrodynamic Cryoprinting of Micropatterned Porous Fiber Scaffolds with Improved MSC Therapy for Wound Healing

    DOI: 10.1007/s42765-022-00224-w
  2. JOURNAL OF NANOBIOTECHNOLOGY (2022) IF: 9.429
    Huc-MSC-derived exosomes modified with the targeting peptide of aHSCs for liver fibrosis therapy

    DOI: 10.1186/s12951-022-01636-x

    PMID: 36183106

    		 Sample: HSC-T6 cells
  3. BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
    L-ergothioneine and its combination with metformin attenuates renal dysfunction in type-2 diabetic rat model by activating Nrf2 antioxidant pathway

    DOI: 10.1016/j.biopha.2021.111921

    PMID: 34346315

    		 Sample: kidney
  4. Nutrients (2022) IF: 6.706
    Supplementation of Lycium barbarum Polysaccharide Combined with Aerobic Exercise Ameliorates High-Fat-Induced Nonalcoholic Steatohepatitis via AMPK/PPARα/PGC-1α Pathway

    DOI: 10.3390/nu14153247
    Sample: serum
  5. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021) IF: 6.953
    Lycium barbarum polysaccharide combined with aerobic exercise ameliorated nonalcoholic fatty liver disease through restoring gut microbiota, intestinal barrier and inhibiting hepatic inflammation

    DOI: 10.1016/j.ijbiomac.2021.05.066

    PMID: 33992651

    		 Sample: Tissue homogenate(Liver)
  6. Frontiers in Medicine (2022) IF: 5.058
    Treatment with β-blocker nebivolol ameliorates oxidative stress and endothelial dysfunction in tenofovir-induced nephrotoxicity in rats.

    DOI: 10.3389/fmed.2022.953749
    Sample: renal tissue
  7. FASEB JOURNAL (2020) IF: 5.192
    Pre‐ischemic renal lavage protects against renal ischemia‐reperfusion injury by attenuation of local and systemic inflammatory responses

    DOI: 10.1096/fj.201902943R
    Sample: Plasma
  8. Food & Function (2022) IF: 5.396
    L-Methionine supplementation attenuates high-fat fructose diet-induced non-alcoholic steatohepatitis by modulating lipid metabolism, fibrosis and inflammation in rats

    DOI: 10.1039/D1FO03403K
    Sample: plasma
  9. JOURNAL OF CELLULAR PHYSIOLOGY (2020) IF: 5.546
    Autophagy decreases alveolar epithelial cell injury by regulating the release of inflammatory mediators

    DOI: 10.1002/jcp.29453

    PMID: 31960959

    		 Sample: Cell culture supernatant,Tissue homogenate
  10. Frontiers in Endocrinology (2021) IF: 5.555
    Chondroitin Sulfate Alleviates Diabetic Osteoporosis and Repairs Bone Microstructure via Anti-Oxidation, Anti-Inflammation, and Regulating Bone Metabolism

    DOI: 10.3389/fendo.2021.759843

    PMID: 34777254

    		 Sample: serum
  11. Drug Design Development and Therapy (2021) IF: 4.162
    Prunella vulgaris L. Attenuates Experimental Autoimmune Thyroiditis by Inhibiting HMGB1/TLR9 Signaling

    DOI: 10.2147/DDDT.S325814

    PMID: 34764638

    		 Sample: blood
  12. Drug Design Development and Therapy (2021) IF: 4.162
    Maackiain Protects the Kidneys of Type 2 Diabetic Rats via Modulating the Nrf2/HO-1 and TLR4/NF-κB/Caspase-3 Pathways

    DOI: 10.2147/DDDT.S326975

    PMID: 34703210

    		 Sample: kidney
  13. Scientific Reports (2017) IF: 4.259
    Hydrogen sulfide ameliorates chronic renal failure in rats by inhibiting apoptosis and inflammation through ROS/MAPK and NF- κ B signaling pathways

    DOI: 10.1038/s41598-017-00557-2
    Sample: Tissue homogenate
  14. ENVIRONMENTAL POLLUTION (2018) IF: 4.358
    COPD rat model is more susceptible to cold stress and PM2.5 exposure and the underlying mechanism

    DOI: 10.1016/j.envpol.2018.05.034
    Sample: Tissue homogenate
  15. ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY (2021) IF: 4.86
    Dasatinib mitigates renal fibrosis in a rat model of UUO via inhibition of Src/STAT-3/NF-κB signaling

    DOI: 10.1016/j.etap.2021.103625

    PMID: 33617955

    		 Sample: Cell（kidney）
  16. BIOCHIMIE (2019) IF: 3.362
    TLR2 and TLR4 mediate an activation of adipose tissue renin-angiotensin system induced by uric acid

    DOI: 10.1016/j.biochi.2019.04.013
    Sample: adipocytes
  17. JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY (2022) IF: 3.568
    Examination of antimicrobial effect of fluoxetine in experimental sepsis model: An in vivo study

    DOI: 10.1002/jbt.23240
    Sample: blood
  18. BMC IMMUNOLOGY (2022) IF: 3.594
    NLRP3 inflammasome up-regulates major histocompatibility complex class I expression and promotes inflammatory infiltration in polymyositis

    DOI: 10.1186/s12865-022-00515-2

    PMID: 35965334

    		 Sample: serum,muscle
  19. PHYTOMEDICINE (2018) IF: 3.61
    Epigallocatechin gallate attenuates mitochondrial DNA-induced inflammatory damage in the development of ventilator-induced lung injury

    DOI: 10.1016/j.phymed.2018.05.017
    Sample: Tissue homogenate
  20. LIFE SCIENCES (2020) IF: 3.647
    IFI16 contributes to the pathogenesis of abdominal aortic aneurysm by regulating the caspase-1/IL-1β/MCPIP1 pathway

    DOI: 10.1016/j.lfs.2020.118752

    PMID: 33188834

    		 Sample: Cell culture supernatant
  21. BIOSCIENCE REPORTS (2019) IF: 3.84
    Up-regulated MCPIP1 in abdominal aortic aneurysm is associated with vascular smooth muscle cell apoptosis and MMPs production

    DOI: 10.1042/BSR20191252

    PMID: 31651935

    		 Sample: Serum,Plasma,Cell sample
  22. Annals of Translational Medicine (2021) IF: 3.932
    Phosphorylated nuclear factor erythroid 2-related factor 2 promotes the secretion of C-C motif chemokine ligand 2 and the recruitment of M2 macrophages

    DOI: 10.21037/atm-21-2947
    Sample: H9C2 cell
  23. JOURNAL OF CHEMICAL NEUROANATOMY (2020) IF: 2.353
    MiR-497 promotes microglia activation and proinflammatory cytokines production in chronic unpredictable stress-induced depression via targeting FGF2

    DOI: 10.1016/j.jchemneu.2020.101872

    PMID: 33068702

    		 Sample: Tissue homogenate
  24. ARCHIVES OF PHYSIOLOGY AND BIOCHEMISTRY (2020) IF: 2.575
    Moringa oleifera Lam. leaf extracts reverse metabolic syndrome in Sprague Dawley rats fed high-fructose high fat diet for 60-days

    DOI: 10.1080/13813455.2020.1762661
    Sample: plasma
  25. Diabetes Metabolic Syndrome and Obesity-Targets and Therapy (2020) IF: 2.842
    Activation of Nrf2 Signaling by Apelin Attenuates Renal Ischemia Reperfusion Injury in Diabetic Rats

    DOI: 10.2147/DMSO.S246743

    PMID: 32606875

    		 Sample: Serum
  26. MOLECULES (2017) IF: 2.861
    Two Sulfur Glycoside Compounds Isolated from Lepidium apetalum Willd Protect NRK52e Cells against Hypertonic-Induced Adhesion and Inflammation by Suppressing the MAPK Signaling Pathway and RAAS

    DOI: 10.3390/molecules22111956
    Sample: Cell culture supernatant
  27. Molecular Medicine Reports (2015) IF: 1.554
    Candesartan ameliorates acute myocardial infarction in rats through inducible nitric oxide synthase, nuclear factor‑κB, monocyte chemoattractant protein‑1, activator protein‑1 and restoration of heat shock protein 72

    DOI: 10.3892/mmr.2015.4432
    Sample: Serum
  28. Molecular Medicine Reports (2017) IF: 1.692
    Osthole protects against inflammation in a rat model of chronic kidney failure via suppression of nuclear factor-κB, transforming growth factor-β1 and activation of phosphoinositide 3-kinase/protein kinase B/nuclear factor (erythroid-derived 2)-like 2 sig

    DOI: 10.3892/mmr.2017.7125
    Sample: serum
  29. Molecular Medicine Reports (2018) IF: 1.922
    Neuroprotective effect of tanshinone IIA weakens spastic cerebral palsy through inflammation, p38MAPK and VEGF in neonatal rats

    DOI: 10.3892/mmr.2017.8069
    Sample: hippocampus
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