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Rat MMP-1(Matrix Metalloproteinase 1) ELISA Kit

  • Cat.No.:E-EL-R0617

  • Reactivity: Rat

To Purchase E-EL-R0617

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.16-10 ng/mL
Sensitivity 0.10 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat MMP-1 in samples. No significant cross-reactivity or interference between Rat MMP-1 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat MMP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-1. You can calculate the concentration of Rat MMP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MMP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MMP-1 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 0.50 1.00 4.50 0.50 1.00 4.60
Standard deviation 0.03 0.05 0.14 0.03 0.06 0.20
CV (%) 6.00 5.00 3.11 6.00 6.00 4.35


The recovery of Rat MMP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 91-104 97
EDTA plasma (n=8) 92-103 97
Cell culture media (n=8) 94-105 99


Samples were spiked with high concentrations of Rat MMP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsMMP1, CLG, CLGN, Interstitial Collagenase, Fibroblast Collagenase
Research AreaCancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. Antioxidants (2022) IF: 7.675
    Protective Effects of Alpha-Lipoic Acid against 5-Fluorouracil-Induced Gastrointestinal Mucositis in Rats

    DOI: 10.3390/antiox11101930

    PMID: 36290656

    Sample: serum,stomach,intestine
  2. Antioxidants (2022) IF: 7.675
    Investigation of the Possible Protective Effect of N-Acetylcysteine (NAC) against Irinotecan (CPT-11)-Induced Toxicity in Rats

    DOI: 10.3390/antiox11112219

    Sample: Serum,Stomach,Small Intestine,Liver,Kidney
  3. Bone & Joint Research (2021) IF: 5.853
    PARP-1 inhibition attenuates the inflammatory response in the cartilage of a rat model of osteoarthritis

    DOI: 10.1302/2046-3758.107.BJR-2020-0200.R2

    PMID: 34254815

    Sample: serum
  4. Scientific Reports (2022) IF: 4.38
    Protective effect of ethanolic extract of Echinacea purpurea contained nanoparticles on meniscal/ligamentous injury induced osteoarthritis in obese male rats

    DOI: 10.1038/s41598-022-09380-w

    PMID: 35354886

    Sample: plasma
    Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study

    DOI: 10.1002/ptr.5949

    Sample: Serum
  6. Food & Function (2019) IF: 3.241
    A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese rats

    DOI: 10.1039/C9FO01342C

    Sample: serum
    prevented osteoarthritis cartilage degeneration by attenuating joint inflammation and collagen breakdown in postmenopausal rat model

    DOI: 10.1007/s10787-018-0452-6

    Sample: serum
    ) protected against osteoarthritis by mitigating inflammation and cartilage degradation: a preclinical study

    DOI: 10.1007/s10787-017-0432-2

    Sample: serum
    Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

    DOI: 10.1111/iej.13121

    Sample: Serum
  10. Biomed Research International (2021) IF: 3.411
    Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

    DOI: 10.1155/2021/8884041

    PMID: 33628825

    Sample: Serum,Tissue homogenate(pulp)
    Epicatechin and scopoletin rich Morinda citrifolia (Noni) leaf extract supplementation, mitigated Osteoarthritis via anti‐inflammatory, anti‐oxidative, and anti‐protease pathways

    DOI: 10.1111/jfbc.12755

    Sample: Serum
  12. Australian Endodontic Journal (2022) IF: 1.659
    Does α-lipoic acid therapeutically effective against experimentally induced-acute pulpitis in rats?

    DOI: 10.1111/aej.12618

    PMID: 35290687

    Sample: sera,pulp
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