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Rat MMP-1(Matrix Metalloproteinase 1) ELISA Kit

Cat.No.:E-EL-R0617
Reactivity: Rat

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.16-10 ng/mL
Sensitivity	0.10 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat MMP-1 in samples. No significant cross-reactivity or interference between Rat MMP-1 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat MMP-1 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-1. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-1. You can calculate the concentration of Rat MMP-1 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MMP-1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MMP-1 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	0.50	1.00	4.50	0.50	1.00	4.60
Standard deviation	0.03	0.05	0.14	0.03	0.06	0.20
CV (%)	6.00	5.00	3.11	6.00	6.00	4.35

Recovery

The recovery of Rat MMP-1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	91-104	97
EDTA plasma (n=8)	92-103	97
Cell culture media (n=8)	94-105	99

Linearity

Samples were spiked with high concentrations of Rat MMP-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	MMP1, CLG, CLGN, Interstitial Collagenase, Fibroblast Collagenase
Research Area	Cancer, Cell Biology, Cardiovascular, Signal transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Antioxidants (2022) IF: 7.675
Protective Effects of Alpha-Lipoic Acid against 5-Fluorouracil-Induced Gastrointestinal Mucositis in Rats

DOI: 10.3390/antiox11101930

PMID: 36290656
Sample: serum,stomach,intestine
Antioxidants (2022) IF: 7.675
Investigation of the Possible Protective Effect of N-Acetylcysteine (NAC) against Irinotecan (CPT-11)-Induced Toxicity in Rats

DOI: 10.3390/antiox11112219

Sample: Serum,Stomach,Small Intestine,Liver,Kidney
Bone & Joint Research (2021) IF: 5.853
PARP-1 inhibition attenuates the inflammatory response in the cartilage of a rat model of osteoarthritis

DOI: 10.1302/2046-3758.107.BJR-2020-0200.R2

PMID: 34254815
Sample: serum
Scientific Reports (2022) IF: 4.38
Protective effect of ethanolic extract of Echinacea purpurea contained nanoparticles on meniscal/ligamentous injury induced osteoarthritis in obese male rats

DOI: 10.1038/s41598-022-09380-w

PMID: 35354886
Sample: plasma
PHYTOTHERAPY RESEARCH (2017) IF: 3.092
Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study

DOI: 10.1002/ptr.5949

Sample: Serum
Food & Function (2019) IF: 3.241
A dietary polysaccharide from Eucheuma cottonii downregulates proinflammatory cytokines and ameliorates osteoarthritis-associated cartilage degradation in obese rats

DOI: 10.1039/C9FO01342C

Sample: serum
INFLAMMOPHARMACOLOGY (2018) IF: 3.304
prevented osteoarthritis cartilage degeneration by attenuating joint inflammation and collagen breakdown in postmenopausal rat model

DOI: 10.1007/s10787-018-0452-6

Sample: serum
INFLAMMOPHARMACOLOGY (2018) IF: 3.304
) protected against osteoarthritis by mitigating inflammation and cartilage degradation: a preclinical study

DOI: 10.1007/s10787-017-0432-2

Sample: serum
INTERNATIONAL ENDODONTIC JOURNAL (2019) IF: 3.331
Effects of alpha‐lipoic acid therapy on experimentally induced apical periodontitis: a biochemical, histopathological and micro‐CT analysis

DOI: 10.1111/iej.13121

Sample: Serum
Biomed Research International (2021) IF: 3.411
Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

DOI: 10.1155/2021/8884041

PMID: 33628825
Sample: Serum,Tissue homogenate(pulp)
JOURNAL OF FOOD BIOCHEMISTRY (2019) IF: 1.358
Epicatechin and scopoletin rich Morinda citrifolia (Noni) leaf extract supplementation, mitigated Osteoarthritis via anti‐inflammatory, anti‐oxidative, and anti‐protease pathways

DOI: 10.1111/jfbc.12755

Sample: Serum
Australian Endodontic Journal (2022) IF: 1.659
Does α-lipoic acid therapeutically effective against experimentally induced-acute pulpitis in rats?

DOI: 10.1111/aej.12618

PMID: 35290687
Sample: sera,pulp

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