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Rat MMP-2(Matrix Metalloproteinase 2) ELISA Kit

Cat.No.:E-EL-R0618
Reactivity: Rat

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96T
48T
24T
96T*5
96T*10

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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	0.31-20 ng/mL
Sensitivity	0.19 ng/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat MMP-2 in samples. No significant cross-reactivity or interference between Rat MMP-2 and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat MMP-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-2. You can calculate the concentration of Rat MMP-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MMP-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MMP-2 were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (ng/mL)	1.10	3.00	8.70	1.10	3.20	9.30
Standard deviation	0.10	0.20	0.40	0.10	0.20	0.40
CV (%)	9.09	6.67	4.60	9.09	6.25	4.30

Recovery

The recovery of Rat MMP-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	88-100	93
EDTA plasma (n=8)	89-103	96
Cell culture media (n=8)	86-96	91

Linearity

Samples were spiked with high concentrations of Rat MMP-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	MMP2, CLG4, CLG4A, MMP-II, MONA, TBE-1, Gelatinase A
Research Area	Cancer, Cell Biology, Cardiovascular, Metabolism

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

Antioxidants (2022) IF: 6.313
Protective Effect of Liposomal Epigallocatechin-Gallate in Experimental Gentamicin-Induced Hepatotoxicity

DOI: 10.3390/antiox11020412

PMID: 35204293
Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2021) IF: 6.53
Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway

DOI: 10.1016/j.biopha.2020.111184

PMID: 33418305
Sample: Plasma
Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
Superoxide Dismutase Mimic, MnTE-2-PyP Enhances Rectal Anastomotic Strength in Rats after Preoperative Chemoradiotherapy

DOI: 10.1155/2020/3509859

Sample: colon anastomosis
JOURNAL OF HEART AND LUNG TRANSPLANTATION (2014) IF: 5.611
Effect of chronic left ventricular unloading on myocardial remodeling: Multimodal assessment of two heterotopic heart transplantation techniques

DOI: 10.1016/j.healun.2014.11.015

Sample: Tissue homogenate
Scientific Reports (2022) IF: 4.996
Alleviation of liver cirrhosis and associated portal-hypertension by Astragalus species in relation to their UPLC-MS/MS metabolic profiles: a mechanistic study

DOI: 10.1038/s41598-022-15958-1

PMID: 35831335
Sample: liver
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY (2019) IF: 3.091
Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

DOI: 10.1111/aji.13096

Sample: Serum
Biomed Research International (2021) IF: 3.411
Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

DOI: 10.1155/2021/8884041

PMID: 33628825
Sample: Serum,Tissue homogenate(pulp)
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY (2021) IF: 3.849
Design, in vitro bioactivity and in vivo influence on oxidative stress and matrix metalloproteinases of bioglasses in experimental skin wound

DOI: 10.1016/j.jtemb.2021.126846

PMID: 34438314
Sample: skin
NEUROTOXICITY RESEARCH (2020) IF: 2.992
The Neuroprotective Effect of Mesna on Cisplatin-Induced Neurotoxicity: Behavioral, Electrophysiological, and Molecular Studies

DOI: 10.1007/s12640-020-00315-9

PMID: 33216283
Sample: sciatic nerve
Australian Endodontic Journal (2022) IF: 1.659
Does α-lipoic acid therapeutically effective against experimentally induced-acute pulpitis in rats?

DOI: 10.1111/aej.12618

PMID: 35290687
Sample: sera,pulp

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Reviews/Q&A

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Reviews
Q&A

S**********nSubmitted [ Jul 13 2023 ]

Asked: Hello, I would like to inquire regarding the sample type to be used in this Elisa Kit. I am working working rat cornea. May I know by processing the samples to become tissue homogenate or tissue lysate would yield the best results? Thank you very much.

adminSubmitted [ Jul 13 2023 ]

Answered: Hello Sze-Min Chan.Thanks for your support. This kit is usually used to detect rat serum / plasma, conventional liver and kidney tissue samples. Rat cornea is very special, and we have not verified it yet. It is recommended that testing tissue homogenate supernatant samples.

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