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Rat MMP-2(Matrix Metalloproteinase 2) ELISA Kit

  • Cat.No.:E-EL-R0618

  • Reactivity: Rat

To Purchase E-EL-R0618

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat MMP-2 in samples. No significant cross-reactivity or interference between Rat MMP-2 and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat MMP-2 concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat MMP-2. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat MMP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat MMP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat MMP-2. You can calculate the concentration of Rat MMP-2 in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat MMP-2 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat MMP-2 were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.10 3.00 8.70 1.10 3.20 9.30
Standard deviation 0.10 0.20 0.40 0.10 0.20 0.40
CV (%) 9.09 6.67 4.60 9.09 6.25 4.30


The recovery of Rat MMP-2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 88-100 93
EDTA plasma (n=8) 89-103 96
Cell culture media (n=8) 86-96 91


Samples were spiked with high concentrations of Rat MMP-2 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

SynonymsMMP2, CLG4, CLG4A, MMP-II, MONA, TBE-1, Gelatinase A
Research AreaCancer, Cell Biology, Cardiovascular, Metabolism

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


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  1. Antioxidants (2022) IF: 6.313
    Protective Effect of Liposomal Epigallocatechin-Gallate in Experimental Gentamicin-Induced Hepatotoxicity

    DOI: 10.3390/antiox11020412

    PMID: 35204293

    Sample: serum
    Water-soluble alkaloids extracted from Aconiti Radix lateralis praeparata protect against chronic heart failure in rats via a calcium signaling pathway

    DOI: 10.1016/j.biopha.2020.111184

    PMID: 33418305

    Sample: Plasma
  3. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Superoxide Dismutase Mimic, MnTE-2-PyP Enhances Rectal Anastomotic Strength in Rats after Preoperative Chemoradiotherapy

    DOI: 10.1155/2020/3509859

    Sample: colon anastomosis
    Effect of chronic left ventricular unloading on myocardial remodeling: Multimodal assessment of two heterotopic heart transplantation techniques

    DOI: 10.1016/j.healun.2014.11.015

    Sample: Tissue homogenate
  5. Scientific Reports (2022) IF: 4.996
    Alleviation of liver cirrhosis and associated portal-hypertension by Astragalus species in relation to their UPLC-MS/MS metabolic profiles: a mechanistic study

    DOI: 10.1038/s41598-022-15958-1

    PMID: 35831335

    Sample: liver
    Nisin reduces uterine inflammation in rats by modulating concentrations of pro‐ and anti‐inflammatory cytokines

    DOI: 10.1111/aji.13096

    Sample: Serum
  7. Biomed Research International (2021) IF: 3.411
    Anti-Inflammatory Effects of Melatonin and 5-Methoxytryptophol on Lipopolysaccharide-Induced Acute Pulpitis in Rats

    DOI: 10.1155/2021/8884041

    PMID: 33628825

    Sample: Serum,Tissue homogenate(pulp)
    Design, in vitro bioactivity and in vivo influence on oxidative stress and matrix metalloproteinases of bioglasses in experimental skin wound

    DOI: 10.1016/j.jtemb.2021.126846

    PMID: 34438314

    Sample: skin
    The Neuroprotective Effect of Mesna on Cisplatin-Induced Neurotoxicity: Behavioral, Electrophysiological, and Molecular Studies

    DOI: 10.1007/s12640-020-00315-9

    PMID: 33216283

    Sample: sciatic nerve
  10. Australian Endodontic Journal (2022) IF: 1.659
    Does α-lipoic acid therapeutically effective against experimentally induced-acute pulpitis in rats?

    DOI: 10.1111/aej.12618

    PMID: 35290687

    Sample: sera,pulp
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  • Q&A

S**********nSubmitted [ Jul 13 2023 ]

Asked: Hello, I would like to inquire regarding the sample type to be used in this Elisa Kit. I am working working rat cornea. May I know by processing the samples to become tissue homogenate or tissue lysate would yield the best results? Thank you very much.



adminSubmitted [ Jul 13 2023 ]

Answered: Hello Sze-Min Chan.Thanks for your support. This kit is usually used to detect rat serum / plasma, conventional liver and kidney tissue samples. Rat cornea is very special, and we have not verified it yet. It is recommended that testing tissue homogenate supernatant samples.

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