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Rat NSE(Neuron Specific Enolase) ELISA Kit

  • Cat.No.:E-EL-R0058

  • Reactivity: Rat

To Purchase E-EL-R0058

  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495

Product Details


Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.31-20 ng/mL
Sensitivity 0.19 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat NSE in samples. No significant cross-reactivity or interference between Rat NSE and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat NSE concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat NSE. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat NSE and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat NSE, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat NSE. You can calculate the concentration of Rat NSE in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data


Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat NSE were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat NSE were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.10 2.20 9.10 1.20 2.30 10.00
Standard deviation 0.10 0.10 0.30 0.10 0.10 0.40
CV (%) 9.09 4.55 3.30 8.33 4.35 4.00


The recovery of Rat NSE spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 94-108 100
EDTA plasma (n=8) 91-105 97
Cell culture media (n=8) 91-105 96


Samples were spiked with high concentrations of Rat NSE and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results


  1. ACS Nano (2023) IF: 18.027
    A Nanotherapy of Octanoic Acid Ameliorates Cardiac Arrest/Cardiopulmonary Resuscitation-Induced Brain Injury via RVG29- and Neutrophil Membrane-Mediated Injury Relay Targeting

    DOI: 10.1021/acsnano.2c09931

    PMID: 36758159

    Sample: serum
  2. Journal of the American Heart Association (2022) IF: 6.106
    Inhaled Carbon Dioxide Improves Neurological Outcomes by Downregulating Hippocampal Autophagy and Apoptosis in an Asphyxia‐Induced Cardiac Arrest and Resuscitation Rat Model

    DOI: 10.1161/JAHA.122.027685

    Sample: blood
  3. Oxidative Medicine and Cellular Longevity (2020) IF: 5.076
    Neuroprotective Effect of the Inhibitor Salubrinal after Cardiac Arrest in a Rodent Model

    DOI: 10.1155/2020/7468738

    PMID: 32064028

    Sample: Serum
  4. Journal of the American Heart Association (2020) IF: 4.605
    Cerebral Blood Flow–Guided Manipulation of Arterial Blood Pressure Attenuates Hippocampal Apoptosis After Asphyxia‐Induced Cardiac Arrest in Rats

    DOI: 10.1161/JAHA.120.016513

    Sample: serum
    Combination of puerarin and tanshinone IIA alleviates ischaemic stroke injury in rats via activating the Nrf2/ARE signalling pathway

    DOI: 10.1080/13880209.2022.2070221

    PMID: 35635784

    Sample: serum
    Triptolide Improves Cognitive Dysfunction in Rats with Vascular Dementia by Activating the SIRT1/PGC-1α Signaling Pathway

    DOI: 10.1007/s11064-019-02831-3

    Sample: serum
    Mitochondrial dynamics and protective effects of a mitochondrial division inhibitor, Mdivi-1, in lipopolysaccharide-induced brain damage

    DOI: 10.1016/j.bbrc.2018.01.136

    Sample: plasma
  8. BMC NEUROSCIENCE (2020) IF: 2.811
    Cathodal tDCS exerts neuroprotective effect in rat brain after acute ischemic stroke.

    DOI: 10.1186/s12868-020-00570-8

    Sample: Serum
    Electroacupuncture reduces astrocyte number and oxidative stress in aged rats with surgery-induced cognitive dysfunction:

    DOI: 10.1177/0300060519860026

    Sample: Serum
    Protective effect of S-allyl cysteine against cerebral ischemia/reperfusion injury in experimental rats

    DOI: 10.4314/tjpr.v20i9.16

    Sample: brain
  11. Neurosciences (2018) IF: 0.929
    Protective role of astragalus injection in spinal cord ischemia-reperfusion injury in rats.

    DOI: 10.17712/nsj.2018.4.20170391

    Sample: serum
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Verified Customer

K**************lSubmitted [ Mar 05 2021 ]

  • Species:Human
  • Sample:Blood serum
  • Sample source:rat blood
  • Detection result:OK
  • Description:We collected blood during experiment and used whole Serum in Assay. Everything went fine using the protocoll
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