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Rat OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit (E-EL-R0710)

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All Size Price Qty
96T $ 495.00
48T $ 396.00
24T $ 150.00
96T*5 Inquire /
96T*10 Inquire /
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For research use only.

Product Summary
Sensitivity 0.28 ng/mL
Detection Range 0.47-30 ng/mL
Sample Volume 100 μL
Total Assay Time 3 h 30 min
Reacitivity Rat
Specificity This kit recognizes Rat OxLDL in samples. No significant cross-reactivity or interference between Rat OxLDL and analogues was observed
Recovery 80%-120%
Sample Type Serum, plasma and other biological fluids
Detection Method Colorimetric method, ELISA, Sandwich
Assay Type Sandwich-ELISA
Size 96T / 48T / 24T / 96T*5 / 96T*10
Storage 2-8℃
Expiration Date 12 months
This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OxLDL. You can calculate the concentration of Rat OxLDL in the samples by comparing the OD of the samples to the standard curve.
Low-density lipoprotein is a kind of lipoprotein particles that carry cholesterol into peripheral tissue cells, which can be oxidized into oxidized low-density lipoprotein. When low-density lipoprotein, especially oxidized low-density lipoprotein (OX-LDL), is excessive, the cholesterol it carries will accumulate in the artery wall, which is easy to cause arteriosclerosis for a long time. That's why LDL is called the "bad cholesterol.
Research Area Cancer , Cardiovascular , Metabolism , Signal Transduction
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