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Rat OxLDL(Oxidized Low Density Lipoprotein) ELISA Kit

  • Cat.No.:E-EL-R0710

  • Reactivity: Rat

To Purchase E-EL-R0710

Size:
  • 96T
  • 48T
  • 24T
  • 96T*5
  • 96T*10
Price: $495
Qty:

Product Details

Properties

Assay type Sandwich-ELISA
Format 96T/48T
Assay time 3.5h
Detection range 0.47-30 ng/mL
Sensitivity 0.28 ng/mL
Sample type &Sample volume serum, plasma and other biological fluids; 100μL
Specificity This kit recognizes Rat OxLDL in samples. No significant cross-reactivity or interference between Rat OxLDL and analogues was observed.
Reproducibility Both intra-CV and inter-CV are < 10%.
Application This ELISA kit applies to the in vitro quantitative determination of Rat OxLDL concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat OxLDL. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat OxLDL and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat OxLDL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat OxLDL. You can calculate the concentration of Rat OxLDL in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.
ItemSpecificationsStorage
Micro ELISA Plate(Dismountable) 96T: 8 wells ×12 strips
48T: 8 wells ×6 strips
-20℃, 6 months
Reference Standard 96T: 2 vials
48T: 1 vial
Concentrated Biotinylated Detection Ab (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×) 96T: 1 vial, 120 μL
48T: 1 vial, 60 μL
-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent 1 vial, 20 mL 2-8°C, 6 months
Biotinylated Detection Ab Diluent 1 vial, 14 mL
HRP Conjugate Diluent 1 vial, 14 mL
Concentrated Wash Buffer (25×) 1 vial, 30 mL
Substrate Reagent 1 vial, 10 mL 2-8℃(Protect from light)
Stop Solution 1 vial, 10 mL 2-8°C
Plate Sealer 5 pieces
Manual 1 copy
Certificate of Analysis 1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat OxLDL were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat OxLDL were tested on 3 different plates, 20 replicates in each plate.

  Intra-assay Precision Inter-assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (ng/mL) 1.60 2.60 12.20 1.50 2.50 13.00
Standard deviation 0.10 0.10 0.50 0.10 0.10 0.40
CV (%) 6.25 3.85 4.10 6.67 4.00 3.08

Recovery

The recovery of Rat OxLDL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type Range (%) Average Recovery (%)
Serum(n=8) 95-107 102
EDTA plasma (n=8) 89-104 96
Cell culture media (n=8) 91-103 97

Linearity

Samples were spiked with high concentrations of Rat OxLDL and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Assay Procedures

elisa assay procedure 1

1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C

elisa assay procedure 2

2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C

elisa assay procedure 3

3. Aspirate and wash the plate for 3 times

elisa assay procedure 4

4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times

elisa assay procedure 5

5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C

elisa assay procedure 6

6. Add 50μL Stop Solution

elisa assay procedure 7

7. Read the plate at 450nm immediately. Calculation of the results

Citations

From now on, if you have published a paper by using any of our products since 1/1/2019, fill out the “Elabscience Publication Reward Application Form”carefully and send it to orders@elabscience.com, we will get back to you with the reward after we confirm it ASAP!

View more details about our Publication Reward >>

  1. ANTIOXIDANTS & REDOX SIGNALING (2022) IF: 7.468
    PRDX6 promotes fatty acid oxidation via PLA2-dependent PPARα activation in rats fed high-fat diet

    DOI: 10.1089/ars.2022.0065

    Sample: serum
  2. Nutrients (2021) IF: 5.719
    The TOTUM-63 Supplement and High-Intensity Interval Training Combination Limits Weight Gain, Improves Glycemic Control, and Influences the Composition of Gut Mucosa-Associated Bacteria in Rats on a High Fat Diet

    DOI: 10.3390/nu13051569

    PMID: 34066988

    Sample: Plasma
  3. Nutrients (2021) IF: 5.719
    Beneficial Effects of High Intensity Interval Training and/or Linseed Oil Supplementation to Limit Obesity-Induced Oxidative Stress in High Fat Diet-Fed Rats

    DOI: 10.3390/nu13103531

    PMID: 34684532

    Sample: plasma
  4. Pharmaceuticals (2021) IF: 5.863
    A Novel Combination Therapy Using Rosuvastatin and Lactobacillus Combats Dextran Sodium Sulfate-Induced Colitis in High-Fat Diet-Fed Rats by Targeting the TXNIP/NLRP3 Interaction and Influencing Gut Microbiome Composition

    DOI: 10.3390/ph14040341

    Sample: serum
  5. Oxidative Medicine and Cellular Longevity (2016) IF: 4.492
    Açai (Euterpe oleracea Mart.) Upregulates Paraoxonase 1 Gene Expression and Activity with Concomitant Reduction of Hepatic Steatosis in High-Fat Diet-Fed Rats

    DOI: 10.1155/2016/8379105

    Sample: Serum
  6. Oxidative Medicine and Cellular Longevity (2019) IF: 4.868
    Tissue-Specific Oxidative Stress Modulation by Exercise: A Comparison between MICT and HIIT in an Obese Rat Model

    DOI: 10.1155/2019/1965364

    PMID: 31396298

    Sample: Plasma,Tissue homogenate
  7. Drug Design Development and Therapy (2015) IF: 3.028
    Edible bird’s nest attenuates procoagulation effects of high-fat diet in rats

    DOI: 10.2147/DDDT.S87772

    Sample: Serum
  8. PHYTOTHERAPY RESEARCH (2019) IF: 3.766
    Synergistic effects of the capsaicinoid nonivamide and rosuvastatin on obesity‐related endothelial dysfunction in rat fed a high‐fat diet

    DOI: 10.1002/ptr.6369

    Sample: blood
  9. Food & Nutrition Research (2015) IF: 2.162
    N-Acetylneuraminic acid attenuates hypercoagulation on high fat diet-induced hyperlipidemic rats

    DOI: 10.3402/fnr.v59.29046

    Sample: Serum
  10. Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
    Edible Bird’s Nest Regulates Hepatic Cholesterol Metabolism through Transcriptional Regulation of Cholesterol Related Genes

    DOI: 10.1155/2022/8882993

    Sample: serum
  11. Anatomy & Cell Biology (2019)
    The effect of alpha-lipoic acid on expression of VCAM-1 in type 2 diabetic rat

    DOI: 10.5115/acb.2019.52.2.176

    Sample: serum
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