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Rat PⅠNP(Procollagen Ⅰ N-Terminal Propeptide) ELISA Kit

Cat.No.:E-EL-R1414
Reactivity: Rat

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96T
48T
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96T*5
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Product Details

Properties

Assay type	Sandwich-ELISA
Format	96T/48T
Assay time	3.5h
Detection range	62.50-4000 pg/mL
Sensitivity	37.50 pg/mL
Sample type &Sample volume	serum, plasma and other biological fluids; 100μL
Specificity	This kit recognizes Rat PⅠNP in samples. No significant cross-reactivity or interference between Rat PⅠNP and analogues was observed.
Reproducibility	Both intra-CV and inter-CV are < 10%.
Application	This ELISA kit applies to the in vitro quantitative determination of Rat PⅠNP concentrations in serum, plasma and other biological fluids.

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat PⅠNP. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat PⅠNP and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat PⅠNP, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat PⅠNP. You can calculate the concentration of Rat PⅠNP in the samples by comparing the OD of the samples to the standard curve.

Kit components & Storage

An unopened kit can be stored at 2-8℃ for 1 month. If the kit is not supposed to be used within 1 month, store the components separately according to the following conditions once the kit is received.

Item	Specifications	Storage
Micro ELISA Plate(Dismountable)	96T: 8 wells ×12 strips 48T: 8 wells ×6 strips	-20℃, 6 months
Reference Standard	96T: 2 vials 48T: 1 vial
Concentrated Biotinylated Detection Ab (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL
Concentrated HRP Conjugate (100×)	96T: 1 vial, 120 μL 48T: 1 vial, 60 μL	-20℃(Protect from light), 6 months
Reference Standard & Sample Diluent	1 vial, 20 mL	2-8°C, 6 months
Biotinylated Detection Ab Diluent	1 vial, 14 mL
HRP Conjugate Diluent	1 vial, 14 mL
Concentrated Wash Buffer (25×)	1 vial, 30 mL
Substrate Reagent	1 vial, 10 mL	2-8℃(Protect from light)
Stop Solution	1 vial, 10 mL	2-8°C
Plate Sealer	5 pieces
Manual	1 copy
Certificate of Analysis	1 copy

Technical Data

Precision

Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Rat PⅠNP were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Rat PⅠNP were tested on 3 different plates, 20 replicates in each plate.

	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean (pg/mL)	211.34	396.29	1351.60	208.17	360.15	1320.24
Standard deviation	12.98	17.24	62.04	12.28	15.41	60.73
CV (%)	6.14	4.35	4.59	5.90	4.28	4.60

Recovery

The recovery of Rat PⅠNP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range (%)	Average Recovery (%)
Serum(n=8)	85-99	90
EDTA plasma (n=8)	89-103	95
Cell culture media (n=8)	89-102	95

Linearity

Samples were spiked with high concentrations of Rat PⅠNP and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

Target Information

Synonyms	P1NP, N-Propeptide Of Type I Procollagen, Procollagen I Amino Terminal Propeptide
Research Area	Signal Transduction

Assay Procedures

	1. Add 100μL standard or sample to the wells. Incubate for 90 min at 37°C
	2. Discard the liquid, immediately add 100μL Biotinylated Detection Ab working solution to each well. Incubate for 60 min at 37°C
	3. Aspirate and wash the plate for 3 times
	4. Add 100μL HRP conjugate working solution. Incubate for 30 min at 37°C. Aspirate and wash the plate for 5 times
	5. Add 90μL Substrate Reagent. Incubate for 15 min at 37°C
	6. Add 50μL Stop Solution
	7. Read the plate at 450nm immediately. Calculation of the results

Citations

JOURNAL OF NANOBIOTECHNOLOGY (2021) IF: 10.435
Remote-controllable bone-targeted delivery of estradiol for the treatment of ovariectomy-induced osteoporosis in rats

DOI: 10.1186/s12951-021-00976-4

PMID: 34407835
Sample: serum
Frontiers in Endocrinology (2022) IF: 6.055
A novel extraction method enhanced the osteogenic and anti-osteoporosis effect of tea extract without any hepatotoxicity in ovariectomized rats.

DOI: 10.3389/fendo.2022.951800

Sample: serum
BIOCHEMICAL PHARMACOLOGY (2022) IF: 6.1
Pterostilbene-isothiocyanate impedes RANK/TRAF6 interaction to inhibit osteoclastogenesis, promoting osteogenesis in vitro and alleviating glucocorticoid induced osteoporosis in rats

DOI: 10.1016/j.bcp.2022.115284

PMID: 36209841
Sample: serum
Scientific Reports (2016) IF: 5.228
Necrostatin-1 treatment inhibits osteocyte necroptosis and trabecular deterioration in ovariectomized rats

DOI: 10.1038/srep33803

Sample: Serum
Cell Death & Disease (2016) IF: 5.378
Loss of Rictor with aging in osteoblasts promotes age-related bone loss

DOI: 10.1038/cddis.2016.249

Sample: Serum
CELLULAR PHYSIOLOGY AND BIOCHEMISTRY (2018) IF: 5.5
Renal Denervation Effects on Myocardial Fibrosis and Ventricular Arrhythmias in Rats with Ischemic Cardiomyopathy

DOI: 10.1159/000489653

Sample: Plasma
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS (2021) IF: 5.571
Cyclodextrin based bone regenerative inclusion complex for resveratrol in postmenopausal osteoporosis

DOI: 10.1016/j.ejpb.2021.07.008

PMID: 34329710
Sample: serum
BONE (2020) IF: 4.147
miR300 intervenes Smad3/β-catenin/RunX2 crosstalk for therapy with an alternate function as indicative biomarker in osteoporosis

DOI: 10.1016/j.bone.2020.115603

Sample: serum
BONE (2020) IF: 4.147
Calcium repletion to rats with calcipenic rickets fails to recover bone quality: A calcipenic “memory”

DOI: 10.1016/j.bone.2020.115562

Sample: serum
BIOMEDICINE & PHARMACOTHERAPY (2020) IF: 4.545
A prebiotic, short-chain fructo-oligosaccharides promotes peak bone mass and maintains bone mass in ovariectomized rats by an osteogenic mechanism

DOI: 10.1016/j.biopha.2020.110448

PMID: 32776872
Sample: Serum
PHYTOTHERAPY RESEARCH (2017) IF: 3.092
Scopoletin‐standardized Morinda elliptica leaf extract suppressed inflammation and cartilage degradation to alleviate osteoarthritis: A preclinical study

DOI: 10.1002/ptr.5949

Sample: Serum
INFLAMMOPHARMACOLOGY (2018) IF: 3.304
) protected against osteoarthritis by mitigating inflammation and cartilage degradation: a preclinical study

DOI: 10.1007/s10787-017-0432-2

Sample: serum
JOURNAL OF ETHNOPHARMACOLOGY (2020) IF: 3.69
QingYan formula extracts protect against postmenopausal osteoporosis in ovariectomized rat model via active ER-dependent MEK/ERK and PI3K/Akt signal pathways

DOI: 10.1016/j.jep.2020.113644

PMID: 33264660
Sample: serum
NUTRITION RESEARCH (2014) IF: 2.585
Vitamin K3 increased BMD at 1 and 2 months post-surgery and the maximum stress of the middle femur in the rat

DOI: 10.1016/j.nutres.2014.10.008

Sample: Serum
Evidence-based Complementary and Alternative Medicine (2022) IF: 2.63
Jiawei Yanghe Decoction Regulates Bone-Lipid Balance through the BMP-SMAD Signaling Pathway to Promote Osteogenic Differentiation of Bone Mesenchymal Stem Cells

DOI: 10.1155/2022/2885419

PMID: 35769158
Sample: plasma
MENOPAUSE-THE JOURNAL OF THE NORTH AMERICAN MENOPAUSE SOCIETY (2018) IF: 2.673
Orthosiphon stamineus (Misai Kucing) ameliorated postmenopausal osteoporosis in rat model

DOI: 10.1097/GME.0000000000000980

Sample: serum
EXPERIMENTAL BIOLOGY AND MEDICINE (2021) IF: 2.691
Zoledronic acid modulates osteoclast apoptosis through activation of the NF-κB signaling pathway in ovariectomized rats:

DOI: 10.1177/15353702211011052

Sample: Serum
DARU-Journal of Pharmaceutical Sciences (2020) IF: 2.739
Comparison of diclofenac with apigenin-glycosides rich Clinacanthus nutans extract for amending inflammation and catabolic protease regulations in osteoporotic-osteoarthritis rat model

DOI: 10.1007/s40199-020-00343-y

PMID: 32388789
Sample: Serum
JOURNAL OF BIOSCIENCES (2019) IF: 1.823
Does cartilage ERα overexpression correlate with osteoarthritic chondrosenescence? Indications from Labisia pumila OA mitigation

DOI: 10.1007/s12038-019-9907-1

Sample: serum

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