Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit (E-CL-R0589)

Name: Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0589
Price: 180.0 USD
Availability: InStock

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Rat RUNX2 (Runt Related Transcription Factor 2) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat RUNX2 in samples.No significant cross-reactivity or interference between Rat RUNX2 and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	57838.000	70208.000	64023.000	63995
2000	28333.000	30179.000	29256.000	29228
1000	14437.000	13575.000	14006.000	13978
500	6892.000	7452.000	7172.000	7144
250	3507.000	3233.000	3370.000	3342
125	1841.000	1589.000	1715.000	1687
62.5	772.000	780.000	776.000	748
0	27.000	29.000	28.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	192.140	626.830	1569.760	187.480	569.380	1437.640
Standard deviation	19.160	51.150	106.430	22.890	47.030	98.620
CV（%）	9.970	8.160	6.780	12.210	8.260	6.860

Recovery

The recovery of Rat RUNX2 was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	96-111	104
EDTA plasma （n=8）	92-105	100
Cell culture media （n=8）	86-100	93

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat RUNX2 and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	97-111	87-103	94-107
1:2	Average	104	94	100
1:4	Range	99-115	97-109	99-112
1:4	Average	106	103	105
1:8	Range	92-104	93-106	97-113
1:8	Average	98	99	104

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat RUNX2. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat RUNX2 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat RUNX2, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat RUNX2. You can calculate the concentration of Rat RUNX2 in the samples by comparing the RLU value of the samples to the standard curve.

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