Rat S100B (S100 Calcium Binding Protein B) CLIA Kit (E-CL-R0591)

Name: Rat S100B (S100 Calcium Binding Protein B) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0591
Price: 180.0 USD
Availability: InStock

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Rat S100B (S100 Calcium Binding Protein B) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	9.38 pg/mL
Detection Range	15.63-1000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat S100B in samples.No significant cross-reactivity or interference between Rat S100B and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
1000	52776.000	61610.000	57193.000	57155
500	23061.000	25999.000	24530.000	24492
250	11042.000	10100.000	10571.000	10533
125	4738.000	5212.000	4975.000	4937
62.5	2617.000	2375.000	2496.000	2458
31.25	1389.000	1373.000	1381.000	1343
15.63	681.000	777.000	729.000	691
0	37.000	39.000	38.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	52.590	127.890	371.750	50.750	127.490	385.210
Standard deviation	6.820	14.000	23.570	6.350	13.090	33.780
CV（%）	12.970	10.950	6.340	12.510	10.270	8.770

Recovery

The recovery of Rat S100B was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	89-106	97
EDTA plasma （n=8）	96-110	102
Cell culture media （n=8）	98-112	104

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat S100B and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	88-101	95-110	90-101
1:2	Average	94	101	95
1:4	Range	89-100	92-108	103-118
1:4	Average	94	99	110
1:8	Range	93-106	90-105	99-113
1:8	Average	100	96	106

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat S100B. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat S100B and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat S100B, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat S100B. You can calculate the concentration of Rat S100B in the samples by comparing the RLU value of the samples to the standard curve.

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