Rat SDF-1α(Stromal Cell Derived Factor 1α)CLIA Kit (E-CL-R0751)

Name: Rat SDF-1α(Stromal Cell Derived Factor 1α)CLIA Kit
Brand: Elabscience
SKU: E-CL-R0751
Price: 180.0 USD
Availability: InStock

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Rat SDF-1α(Stromal Cell Derived Factor 1α)CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	4.69 pg/mL
Detection Range	7.81-500 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat SDF-1α in samples.No significant cross-reactivity or interference between Rat SDF-1α and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
500	51569.000	60645.000	56107.000	56073
250	20873.000	21919.000	21396.000	21362
125	10831.000	9667.000	10249.000	10215
62.5	4383.000	4741.000	4562.000	4528
31.25	2419.000	2215.000	2317.000	2283
15.63	1326.000	1238.000	1282.000	1248
7.81	730.000	790.000	760.000	726
0	33.000	35.000	34.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	23.440	57.840	230.700	21.510	54.780	213.080
Standard deviation	2.300	4.200	23.880	2.550	5.410	16.810
CV（%）	9.810	7.260	10.350	11.850	9.880	7.890

Recovery

The recovery of Rat SDF-1α was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	94-107	99
EDTA plasma （n=8）	84-100	91
Cell culture media （n=8）	94-105	100

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat SDF-1α and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	91-103	95-112	92-104
1:2	Average	98	102	97
1:4	Range	89-106	93-104	101-115
1:4	Average	97	99	109
1:8	Range	99-110	96-112	85-95
1:8	Average	105	102	90

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat SDF-1α. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDF-1α and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDF-1α, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat SDF-1α. You can calculate the concentration of Rat SDF-1α in the samples by comparing the RLU value of the samples to the standard curve.

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