Product Summary

Sensitivity	1.88 ng/mL
Detection Range	3.13-200 ng/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat sIgA in samples. No significant cross-reactivity or interference between Rat sIgA and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Colorimetric method, ELISA, Sandwich
Assay Type	Sandwich-ELISA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃
Expiration Date	12 months

Test Principle

This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat sIgA. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat sIgA and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat sIgA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat sIgA. You can calculate the concentration of Rat sIgA in the samples by comparing the OD of the samples to the standard curve.

Background

he secreted component is A single chain glycoprotein that can exist freely or as a component of secreted immunoglobulin A (SIgA). IgA regulates the physiological balance between symbiotic gut bacteria and host immune system defenses. It is the same type that produces the most antibodies. IgAs are secreted mainly in the intestinal mucosa to prevent symbiotic microorganisms from invading the intestine

Research Area

Cancer , Immunology