Rat TAT (Thrombin-Antithrombin Complex) CLIA Kit (E-CL-R0637)

Name: Rat TAT (Thrombin-Antithrombin Complex) CLIA Kit
Brand: Elabscience
SKU: E-CL-R0637
Price: 180.0 USD
Availability: InStock

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Rat TAT (Thrombin-Antithrombin Complex) CLIA Kit - 1

Size	Price	Qty
96T	$ 682.00	- +
48T	$ 546.00	- +
24T	$ 180.00	- +
96T*5	Inquire	/
96T*10	Inquire	/

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Product Summary

Sensitivity	37.5 pg/mL
Detection Range	62.5-4000 pg/mL
Sample Volume	100 μL
Total Assay Time	3 h 30 min
Reactivity	Rat
Specificity	This kit recognizes Rat TAT in samples.No significant cross-reactivity or interference between Rat TAT and analogues was observed
Recovery	80%-120%
Sample Type	Serum, plasma and other biological fluids
Detection Method	Chemiluminescence
Assay Type	Sandwich-CLIA
Size	96T / 48T / 24T / 96T5 / 96T10
Storage	2-8℃/-20℃
Expiration Date	6 months

Performance

Typical data

The following data was generated by the Quality Control Department, under controlled laboratory conditions (ambient temperature: 18-25 °C, relative humidity: 35-75%) using standardized procedures (TMB reaction at 37 °C in the dark for 15 minutes, followed by termination and OD measurement). These values are provided for reference only. Actual results may vary due to differences in laboratory conditions, operator technique, and equipment. Users are required to generate a standard curve using their own experimental data.

pg/mL	OD1	OD2	Mean OD	Corrected OD
4000	59345.000	68135.000	63740.000	63709
2000	28450.000	28556.000	28503.000	28472
1000	15512.000	14096.000	14804.000	14773
500	6742.000	7272.000	7007.000	6976
250	3365.000	3241.000	3303.000	3272
125	1703.000	1485.000	1594.000	1563
62.5	766.000	830.000	798.000	767
0	30.000	32.000	31.000	-

Precision

Intra-assay Precision (Within-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested 20 times on a single plate.

Inter-assay Precision (Between-run Precision): Three samples representing low, mid, and high concentrations of Human IL-6 were tested on three separate plates, with 20 replicates per plate, to assess variability among assays.

/	Intra-assay Precision			Inter-assay Precision
Sample	1	2	3	1	2	3
Numbers	20	20	20	20	20	20
Mean（pg/mL）	209.060	545.430	1341.660	196.470	567.360	1329.340
Standard deviation	24.150	42.000	100.890	23.990	45.220	128.150
CV（%）	11.550	7.700	7.520	12.210	7.970	9.640

Recovery

The recovery of Rat TAT was evaluated by spiking samples at low, mid, and high concentrations across the assay range in various sample matrices.
The assay performance was assessed by comparing the measured concentrations to the expected spiked amounts to determine the percent recovery.

Sample Type	Range （%）	Average Recovery（%）
Serum （n=8）	91-105	96
EDTA plasma （n=8）	100-117	107
Cell culture media （n=8）	90-103	95

Linearity

Linearity of the assay was evaluated by spiking samples with high concentrations of Rat TAT and performing serial dilutions using Standard & Sample Diluent to produce concentrations spanning the assay's dynamic range. The measured values were then compared to the expected concentrations to assess the linearity of response.

/	/	Cell culture media （n=5）	EDTA plasma （n=5）	Serum （n=5）
1:2	Range	92-102	91-102	89-103
1:2	Average	97	97	96
1:4	Range	93-108	94-108	88-100
1:4	Average	99	101	93
1:8	Range	101-115	99-115	88-101
1:8	Average	107	105	94

Test Principle

This CLIA kit uses the Sandwich-CLIA principle. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to Rat TAT. Samples or Standards are added to the micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat TAT and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat TAT, biotinylated detection antibody and Avidin-HRP conjugate will appear luminescence. The Relative light unit (RLU) value is measured by the Chemiluminescence immunoassay analyzer at 425nm. The RLU value is positively associated with the concentration of Rat TAT. You can calculate the concentration of Rat TAT in the samples by comparing the RLU value of the samples to the standard curve.

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